2型糖尿病大鼠近球小管Na^+,K^+-ATPase活性变化  

Changes of Na^+,K^+-ATPase activity in proximal tubules of type 2 diabetic rats

作　　者：刘红[1] 罗蕾[1] 高原[1] 

机构地区：[1]遵义医学院珠海校区生理学教研室,珠海519041

出　　处：《第三军医大学学报》2006年第20期2062-2064,共3页Journal of Third Military Medical University

基　　金：珠海市科技基金资助项目(PC2003010057)~~

摘　　要：目的探讨2型糖尿病(type 2 diabetes mellitus,T2DM)近球小管(proximaltubule,PT)的Na^+,K^+-ATPase活性变化。方法首先建立T2DM大鼠模型,测定血糖、血清胰岛素和内源性洋地黄样物质(endogenous digitalis-like sub-stance,EDLS)水平;微分离大鼠单根PT,液闪法测单根PT的Na^+,K^+-ATPase活性。结果与正常对照组大鼠比较,模型组血糖、血脂、总胆固醇浓度显著升高,胰岛素敏感指数显著下降(P<0.05,P<0.01);胰岛素水平不低,PT的Na^+,K^+-ATPase活性均显著升高(P<0.01);血清EDLS水平显著下降(P<0.01)。大鼠血清EDLS水平与PT的Na^+,K^+-AT-Pase活性呈显著负相关(r=-0.900,P<0.01)。结论T2DM大鼠PT的Na^+,K^+-ATPase活性升高,这种酶活性升高与血液中EDLS水平下降有关。Objective To investigate the changes of Na^＋ , K^＋ -ATPase activity in the proximal tubules （PT） of type 2 diabetes mellitus（T2DM） and the correlation of PT Na^＋ , K^＋-ATPase activity and endogenous digitalis-like substance （EDLS）. Methods Female Wistar rats were developed a rat model of T2DM and then divided into 3 groups after injected stretozotocin, that is, 5 weeks group, 7 weeks group and 9 weeks group. control group, T2DM rats were associated with hyperglycemia, hypertriglyceridemia, dyslipidemia, and insulin resistance ; Na ^＋, K^＋ -ATPase activity of PT in T2DM rats was significantly increased, and there was no significant difference among 3 sub-groups. Serum EDLS level was significantly enhanced in T2DM, however, EDLS levels were no significant difference among 3 sub-groups. Na^＋ , K^＋ -ATPase activity of PT had negative relation with EDLS level in serum. Conclusion Na^＋ , K^＋ -ATPase activity of PT in T2DM rats is increased and EDLS level in serum decrease may play a important role.

关 键 词：2型糖尿病 近球小管 Na^+ K^+-ATPase 内源性洋地黄样物质 大鼠 

分 类 号：R322.61[医药卫生—人体解剖和组织胚胎学] R329.26[医药卫生—基础医学]