超声引导经皮兔肝内注射高温蒸馏水加干扰素对肝组织病理及免疫变化的实验研究  

Ultrasound-guided Percutaneous Hyperthermal Distilled Water and Interferon Injection Therapy in Rabbit Liver:Experimeental Study

作　　者：李文伦[1] 黄进[2] 王会东[1] 张华民[1] 张永庆[1] 

机构地区：[1]潍坊市人民医院,山东省潍坊市261041 [2]潍坊市中医院

出　　处：《中国超声医学杂志》2006年第10期733-735,共3页Chinese Journal of Ultrasound in Medicine

基　　金：山东省自然基金资助项目(No.20021106)

摘　　要：目的在超声引导经皮兔肝内注射高温蒸馏水(90℃)加干扰素,分析局部肝组织的病理学及免疫学变化。方法对8只健康家兔肝内分别注射高温蒸馏水2、4、8、16ml,干扰素300万U观察即刻、5、7d的病理学及免疫学变化。结果高温蒸馏水肝内注射后,肝组织早期即出现坏死的形态学改变,坏死区大小随剂量而不同。5、7d后观察,肝组织可见大片凝固性坏死,周边包膜形成,病理凝固区及边缘变性坏死,失去小叶结构,中度水肿,与正常肝组织界限清晰,汇管区均见数量不等的炎细胞浸润;血清IgG及T淋巴细胞转化率升高。结论高温蒸馏水加干扰素致肝坏死确切、有效,能使免疫功能提高,为临床应用提供了理论依据。Objective To analyse the rabbit liver pathological and immunological changes after the ultrasoundguided percutaneous hyperthermal distilled water and interferon injection. Methods All eight healthy rabbit livers were injected hyperthermal distilled water with different volumes, 2, 4, 8 and 16 mls, and 3 million unit interferon. Pathological and immunological changes were observed imediately and on 5 and 7 day. Results At the early stage after the hyperthermal distilled water injection, necrosis appeared in the rabbit liver, the more volumes were injected, the bigger necrosis area appeared. On the 5 and 7 day, massive coagulative necrosis area appeared and normal live lobules disappeared instead of edema and white cell infiltration. At the same time, serum IgG level and T cell translation rate were higher than those before treatment. Conclusions The hyperthermal distilled water combined with interferon injection can induce significant hepatonecrosis and elevate immune reaction, which provides theoratical basis for clinical.

关 键 词：超声引导 高温蒸馏水 干扰素 肝癌治疗 

分 类 号：R96[医药卫生—药理学]