我国不同地域牦牛BLG基因部分核苷酸序列同源性分析  

Homologous Analysis of Partial Nucleotide Sequences of BLG Gene in Yaks From Different Areas in China

作　　者：刘建忠[1] 田为宇[1] 周丽芳[1] 敖敬[1] 

机构地区：[1]武汉科技大学化学工程与技术学院

出　　处：《武汉科技大学学报》2006年第5期469-472,共4页Journal of Wuhan University of Science and Technology

基　　金：国家863计划资助项目(2001AA213081)

摘　　要：设计一对PCR引物,寡聚核苷酸序列的上游引物为5-′gCg,AAT,TCA,TCC,CAC,gTg,CCT,gC-3,′下游引物为5-′gAg,AAT,TCC,Tgg,ggA,ggg,ACC,TT-3′。经68℃退火及30轮循环的PCR程序后,分别克隆了来自我国甘肃、青海和云南地区牦牛β乳球蛋白基因的5′调控区1 447 bp的DNA片段。将该片段从琼脂糖凝胶中回收,克隆在pMD18-T Vector后进行序列测定。序列分析结果表明,甘肃牦牛与青海牦牛该序列的核苷酸同源性为98.13%,与云南牦牛该序列的核苷酸同源性为97.65%,青海牦牛与云南牦牛该基因片段的核苷酸序列同源性为99.45%。In this study a pair of primers for PCR were designed, the oligonucleotides were as follows： the upstream primer, 5＇-gCg, AAT, TCA, TCC, CAC, gTg, CCT, gC-3＇, downstream primer, 5＇-gAg, AAT, TCC, Tgg, ggA, ggg, ACC, TT-3＇. After 30 cycles of PCR procedures with annealing temperature at 68 ℃ , a fragment of the 5＇ regulatory region of β-lactoglobulin gene（1 447 bp） were amplified from Gansu, Qinghai and Yunnan yaks respectively and collected from agarose gel. Then the fragments were cloned into pMD18-T vector and sequenced. The sequencing results show that the homology in the 5＇regulatory region of β-lactoglobulin gene between Gansu and Qinghai yaks is 98.13% , that between Gansu and Yunnan yaks 97.65%, and that between Qinghai and Yunnan yaks as high as 99.45%.

关 键 词：牦牛 BLG基因 同源性分析 

分 类 号：Q75[生物学—分子生物学]