金属离子对皱纹盘鲍组织培养的影响  

Effects of Metal Ions on Tissue Culture of Haliotis discus hannai

作　　者：孙振兴[1] 李清[1] 张丽[1] 马运超[1] 张媛[1] 

机构地区：[1]鲁东大学生命科学学院,山东烟台264025

出　　处：《鲁东大学学报（自然科学版）》2006年第3期224-227,共4页Journal of Ludong University:Natural Science Edition

基　　金：山东省自然科学基金项目(Y2002D12)

摘　　要：采用3因素3水平L9(34)的正交设计,以组织块增殖百分率作为评价指标,研究了在M199和RPMI-1640培养基中分别添加Ca2+,Mg2+,Zn2+金属离子对鲍的外套膜和上足触手组织培养的影响.结果显示,Ca2+和Zn2+对细胞增殖具有明显的促进作用,Mg2+的作用不明显;对上足触手细胞生长最好的组合为5 g/L的Ca2+,1 g/L的Mg2+,20μg/L的Zn2+;对外套膜细胞生长最好的组合为1 g/L的Ca2+,1 g/L的Mg2+,60μg/L的Zn2+.The effects of three metal ions on the cultured tissue from mantle and epipodial tentacle of Haliotis discus hannai with Ca^2＋ , Mg^2＋ and Zn^2＋ ions which are added into the M199 and RPMI- 1640 medium are studied respectively. The experiment is projected according to orthogonal design L9 （3^4）, the proliferation percent of tissue blocks is estimated as the indicator of growth.The results show that the Ca^2＋ and Zn^2＋ have an obvious effect on accelerating the proliferation of cultured cells, but the effect of the Mg^2＋ is not evident. When Ca^2＋ is 5g/L, Mg^2＋ is 1g/L and Zn^2＋ is 20μg/L, the growth of cells of epipodial tentacle is growth conditions of the mantle cells are that the concentration of Ca^2＋ is 1g/L, Mg^2＋ 1g/L the concentration of the best. But the best and Zn^2＋ 60μg/L.

关 键 词：皱纹盘鲍 外套膜 上足触手 组织培养 金属离子 

分 类 号：Q813.1[生物学—生物工程] S917.4[农业科学—水产科学]