虎奶菇菌核蛋白多糖的分离纯化及化学结构分析  

Isolation, Purification and Chemical Structure of a Pleurotus tuber-regium Proteoglycan

作　　者：翁伯琦[1] 江枝和[2] 宾文[3] 王义祥[1] 罗涛[2] 林勇[2] 

机构地区：[1]福建省农业科学院农业生态研究所,福州350013 [2]福建省农业科学院食用菌研究开发中心,福州350013 [3]沈阳药科大学制药工程学院,沈阳110015

出　　处：《食用菌学报》2006年第3期61-69,共9页Acta Edulis Fungi

基　　金：福建省科技厅项目"食药用菌高产栽培技术研究"(2001Z128);"福建省山地草业工程技术研究中心"(2005N029)的部分研究内容

摘　　要：虎奶菇菌核干品经水提、醇沉、Sevage法除蛋白、透析得纯蛋白多糖。分析结果表明,该纯蛋白多糖分子质量为39810,糖含量为61.7%,氮含量为38%,由葡萄糖、半乳糖等单糖组成,糖基连接键以β-1,4为主,还有β-1,3连接键。A proteoglycan with a molecular mass of 39810 as determined by gel filtration, was obtained from dry, powdered sclerotia of Pleurotus tuber-regium following extraction with water, ethanol precipitation, protein removal by the Sevage method, dialysis, and Sephadex G-75 chromatography. Chemical analyses revealed the material to consist of 61.7% total sugar （calculated as glucose） and 38% nitrogen, and gas chromatography of acid-hydrolysis products indicated that the major monosaccharide components were glucose and galactose. Smith degradation data suggested that the main glycosidic linkage in the proteoglycan was β-1,4, and that β-1,3 linkages may also be present.

关 键 词：化学结构 虎奶菇 蛋白多糖 菌核 

分 类 号：S646[农业科学—蔬菜学]