丹参酮对脑梗死患者白细胞表面黏附分子表达的影响  

Role of sulfotanshinone sodium(丹参酮) on polymorphonuclear adhesion molecule expression in cases with acute cerebral infarction

作　　者：李敬诚[1] 周华东[1] 张猛[1] 陈曼娥[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所神经内科脑二科,重庆400042

出　　处：《中国中西医结合急救杂志》2006年第5期263-265,共3页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：国家自然科学基金资助项目(39670268)

摘　　要：目的:通过对白细胞表面黏附分子表达的分析,探讨丹参酮治疗急性脑梗死的作用机制。方法:采用双盲随机对照方法将80例急性脑梗死患者分为两组,治疗组给予丹参酮注射液2 m l,对照组给予注射用水,两组分别溶于生理盐水250 m l静脉滴注,每日1次,连用7 d。两组于治疗前后取外周血分离多形核白细胞(PM N),应用间接免疫荧光标记,流式细胞仪检测白细胞表面黏附分子CD 11a、CD 18、CD 18/CD 11a(LFA 1)免疫阳性细胞数;应用透射电镜观察外周血PM N超微结构变化。结果:丹参酮能明显降低外周血白细胞表面黏附分子CD 11a、CD 18、LFA 1免疫阳性细胞数;PM N超微结构显示,与治疗组比较,对照组胞浆电子密度降低,内质网扩大,部分线粒体嵴断裂,核周间隙增高,核浆比例增大均更加明显。结论:丹参酮抑制白细胞表面黏附分子CD 11a、CD 18、LFA 1的表达,阻断白细胞与血管内皮细胞黏附,在脑梗死治疗中具有保护神经细胞的作用。Objective： To investigate the role of sulfotanshinone sodium （丹参酮） for treatment of acute cerebral infarction （ACI） and its mechanism by accessing the expression of adhesion molecules of polymorphonuclear （PMN） cells. Methods： Eighty adult patients with ACI were randomly divided into sulfotanshinone group and control group according to double-blind, randomized and controlled study. Sulfotanshinone sodium （2 ml） dissolved in 250 ml of normal saline was intravenously dripped once a day in sulfotanshinone group （n = 65）, while water injectio （2 ml） in 250 ml of normal saline was intravenously dripped once a day in control group （n= 15）, the therapeutic course being 7 days. The percentages of CDlla, CD18 and CD18/CD11a （LFA - 1） expression originated from PMN cells were measured with indirect immunofluorescence and flow cytometry, cellular ultrastructures of PMN cells in peripheral blood were observed by a transmission electron microscope before and after treatment and the two groups were compared. Results： Sulfotanshinone sodium could obviously decrease the numbers of immunopositive CD11a, CD18 and LFA - 1 cells in blood. In comparison between the control group and the treatment group, the ultrastructural changes showed that the following items were more marked in the former group than those in the latter group ： decrease in cytoplasmic electron density, dilatation of endoplasmic reticulum, breaks of mitochondria cristae, enlargement of perinuclear space and increase in karyoplasm proportion. Conclusion： Sulfotanshinone sodium may inhibit the expressions of CD11a, CD18 and LFA - 1 adhesion molecules on the surface of white blood cells, prevent the adhesion of the cells onto the endothelium of microvessels and play a protective role on nerve cells in cases with ACI.

关 键 词：脑梗死 丹参酮 细胞黏附分子 

分 类 号：R277.7[医药卫生—中医学]