中国四省甲型副伤寒分离株脉冲场凝胶电泳分析及分型数据库建立  

Molecular typing of Salmonella paratyphi A isolates from four provinces with pulse-field gel electrophoresis

作　　者：李伟[1] 崔志刚[1] 张政[2] 田克诚[3] 钟豪杰[4] 朱凤才[5] 梁未丽[1] 章丽娟[1] 阚飙[1] 

机构地区：[1]中国疾病预防控制中心传染病预防控制所国家重点实验室,北京102206 [2]浙江省疾病预防控制中心 [3]贵州省疾病预防控制中心 [4]广东省疾病预防控制中心 [5]江苏省疾病预防控制中心

出　　处：《中华流行病学杂志》2006年第10期871-874,共4页Chinese Journal of Epidemiology

基　　金：国家"十五"科技攻关课题资助项目(2003BA712A05-04)

摘　　要：目的对中国4个甲型副伤寒流行省份的分离株进行标准化脉冲场凝胶电泳(PFGE)分型分析。方法对来自4个省的甲型副伤寒沙门菌利用XbaⅠ酶切染色体DNA进行PFGE分析,利用BioNumerics建立数据库和进行相似性聚类。结果分离自1998-2002年的89株甲型副伤寒分离株共有11种PFGE带型,这些菌株中具有优势带型。有3种带型相似性在96．3％,这3种带型的菌株占到分析菌株的86．5％,并在不同省份和年度中出现。结论建立了中国甲型副伤寒沙门菌的用于网络监测分析的PFGE技术和数据库,近年中国部分省份的甲型副伤寒沙门菌分离株PFGE型别集中,存在同型菌株的广泛流行。Objective To analyze the molecular types of Salmonella paratyphi A strains isolated in the recent years, and to construct the standard S. paratyphi A databank in the laboratory surveillance network PulseNet China. Methods S. paratyphi A isolates from 4 provinces were analyzed with the standard pulsed-field gel electrophoresis （PFGE） protocol used in PulseNet and their patterns compared. The databank was constructed with BioNumerics. Results Eleven PFGE patterns were obtained, in which 3 predominant patterns were identifies with a similarity coefficient of 96.3%. The strains of these patterns, accounted for 86.5% of the analyzed strains, appeared in different provinces and years. Conclusion The databank of S. paratyphi A was constructed and could be used in laboratory surveillance of S. paratyphi A in PulseNet China. From the analyses on molecular typing of the isolates, data suggested that the predominant strains might cause the epidemics in different regions.

关 键 词：甲型副伤寒沙门菌 脉冲场凝胶电泳 分子分型 

分 类 号：R378[医药卫生—病原生物学]