配位作用毛细管电泳方法研究  

Novel Capillary Electrophoresis Technique Based on Coordination Interaction

作　　者：魏为力[1] 陈志涛[1] 石开云[2] 袁林[2] 蒋雪梅[2] 夏之宁[1] 

机构地区：[1]重庆大学化学化工学院药学系,重庆400044 [2]重庆大学生物工程学院,重庆400044

出　　处：《分析化学》2006年第9期1219-1222,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.20375051);教育部高校青年教师(No.教人司[2002]123)资助项目

摘　　要：研究了一种以过渡金属离子为添加剂，基于配位相互作用的毛细管电泳技术。选择7种芳香胺类化合物作为分析对象，考察了金属离子种类和浓度、运行介质酸度、电压对配位毛细管电泳的影响。通过对邻苯二胺变质样品的鉴别以及实际合成药磺胺类药物的分离，考察了这种毛细管电泳分离技术的分离能力，效果令人满意。应用于颠茄磺苄啶片中磺胺甲嗯唑（SMZ）的定量测定时，SMZ的检出限为0．12mmol／L；线性范围为0．25—10mmol／L（r^2=0．9917）；平均加样回收率为97．8％，表明所建立的配位作用毛细管电泳（CICE）方法，具有稳定、可靠的定性和定量分析能力。A capillary electrophoretic method based on coordination interaction has been developed by utilizing seven aromatic amines as model compounds, in which Cu^2＋ was selected as additive of the running medium. The separation condition and the composition of running medium were optimized. In addition, the limits of detection and linear range of concentration were given for each model compounds. The qualitative and quantitative reliability of the method, as given by resolutions and detection limits, was presented. And the analysis results of decomposed o-penylenediamine sample and sulfonamids are presented.

关 键 词：配位相互作用 毛细管电泳 铜离子 磺胺 

分 类 号：O658.9[理学—分析化学]