藏红T作为电化学探针线扫极谱法测定透明质酸  

Linear Sweep Polarographic Determination of Hyaluronic Acid with Safranine T as Electrochemical Probe

作　　者：孙伟[1] 高瑞芳[1] 丁雅勤[1] 焦奎[1] 

机构地区：[1]青岛科技大学化学与分子工程学院,青岛266042

出　　处：《分析化学》2006年第9期1265-1268,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.20405008;20375020);青岛市自然科学基金(No.04-2-JZ-114);青岛科技大学博士基金(No.0022125)资助项目

摘　　要：在pH1．5的Britton—Robinson（B—R）缓冲溶液中，藏红T（ST）在-0．35V（vs ．SCE）处有一个灵敏的线性扫描二阶导数极谱还原波。当加入一定量的透明质酸（HA）后，由于此条件下ST带正电荷，而HA带负电荷，两者之间通过静电力作用形成一种生物超分子复合物，导致溶液中游离的ST的浓度降低，相应的还原峰电流降低。实验优化了结合反应条件和电化学测定条件。在最佳条件下，还原峰电流的降低值与HA的浓度在3．0～100．0mg／L范围内呈线性关系，线性回归方程为△ip″（nA／s^-2）=27．23＋15．18C（mg／L）（n=14，r=0．996）。本方法成功应用于HA模拟样品的测定。In pH 1.5 Britton-Robinson buffer solution, safranine T （ST） has a sensitive second order derivative linear sweep polarographic reductive wave at -0.35 V （vs. SCE） on drop mercury working electrode. After the addition of hyaluronic acid （HA） to the ST solution, a supramolecular complex was formed based on the electrostatic attraction of positively charged ST with negatively charged HA, which resulted in the decrease of the free concentration of ST in the solution, and the decrease of peak current. The condition of the binding reaction and the electrochemical detection were optimized. Under the optimum conditions, the ip″ of ST decreased proportionally to the HA concentration from 3.0 to 100.0 mg/L with the linear regression equation as △i″p （ nA/s^ -2 ） = 27.23 ＋ 15.18C（mg/L） （ n = 14, r = 0. 996）. The method bas been applied to the determination of HA synthetic samples with satisfactory results and the binding number was calculated as 870 by molar ratio method.

关 键 词：透明质酸 藏红T 极谱分析 结合反应 

分 类 号：O657.14[理学—分析化学]