超支化聚酯化学键合涂层毛细管电泳柱的制备及碱性蛋白质的分离  

Preparation of Chemical-bond Hyperbranched Polyesters Coated Columns for Capillary Electrophoresis and Their Application to Separation of Basic Proteins

作　　者：寿崇琦[1] 张志良[2] 邢希学[1] 康杰分[1] 欧庆瑜[3] 

机构地区：[1]济南大学化学化工学院,济南250022 [2]山东轻工业学院分析测试中心,济南250100 [3]中国科学院兰州化学物理研究所,兰州730000

出　　处：《分析化学》2006年第9期1287-1290,共4页Chinese Journal of Analytical Chemistry

摘　　要：用一步法和准一步法合成了以三羟甲基丙烷为核的两个系列的超支化聚酯，利用红外光谱、羟值测定等手段对其分子结构进行了表征。利用超支化聚合物低粘度的特点，采用化学键合的方法将其涂于石英毛细管电泳柱内壁，使其在毛细管内壁上形成稳定的超支化聚酯涂层。该涂层在pH3．0—7．0范围内能够有效地抑制电渗流和碱性蛋白质在毛细管壁上的吸附。实验结果表明：该涂层柱在pH5．0的磷酸缓冲溶液中，对碱性蛋白质的分离柱效可高达塔板数10^6／m。每次运行之间（n=6），天与天之间（n=3），以及柱与柱之间（N=3）的迁移时间的标准偏差（RSD％）在0．5％-1．5％之间，表明本方法制得的涂层柱具有良好的稳定性。Two series of hyperbranched polyesters with trimethylolpropane as core were synthesized using one-step and pseudo-step procedure, and the molecular structures of them were characterized by infrared spectra （IR） and hydroxyl value. They were coated and formed steady coatings on the inner surface of the fusedsilica capillaries in chemically-bond Way. The coatings could reduce the electroosmotic flow greatly and suppress protein adsorption between the inner surface of the fused-silica capillaries and the basic proteins effectively in the pH range of 3 -7. The separation efficiency of 106 plates/m was obtained. Good reproducibility （ RSD 〈 1.5 % ） of migration time from run to run, day to day and column to column was also obtained.

关 键 词：毛细管电泳 蛋白质 涂层柱 超支化聚酯 

分 类 号：O658.9[理学—分析化学]