天青Ⅰ修饰玻碳电极的电化学性质及其对NADH的催化氧化  

Electrocatalysis of NADH Oxidation at AzureⅠ Chemically Modified Electrodes

作　　者：王娜[1] 袁若[1] 柴雅琴[1] 朱强[1] 黎雪莲[1] 

机构地区：[1]西南师范大学化学化工学院重庆市现代分析化学重点实验室,重庆400715

出　　处：《分析科学学报》2006年第5期537-539,共3页Journal of Analytical Science

基　　金：国家自然科学基金(No.29705001);重庆市自然科学基金(No.CSTC-2004BB4149);西南师范大学博士基金(No.SWNUB2004021)

摘　　要：本文利用滴涂于玻碳表面的Nation膜中负电性的磺酸基与天青Ⅰ阳离子之间的静电作用，以实现天青Ⅰ的固定化，从而制备出Nafion／天青Ⅰ电催化型烟酰胺腺嘌呤二核苷酸（NADH）传感器。采用循环伏安法考察了传感器的电化学性质，并研究了该修饰电极对NADH的电催化作用。实验结果表明：该修饰电极对NADH有良好的电催化作用，NADH氧化峰电位比未修饰的玻碳电极负移了660mV，响应电流与NADH的浓度在8．7×10^-5～1．5×10^-2mol／L范围内呈良好的线性关系。该方法检出限为3．0×10^-5mol／L。The sensor for electrocatalytic oxidation of reduced nicotinamide adenine dinucleotide （NADH） was prepared by immersing a Nation film coated glassy carbon electrode into azure Ⅰ solution. The azure Ⅰ was immobilized via electrostatic force between the cation azure Ⅰ and the negatively charged sulfonic acid groups in Nation film. Cyclic vohammetry （CV） was used to investigate the electrochemical characteristics of the sensor. The experimental results demonstrated that the modified electrode was an excellent catalyst for NADH oxidation, the peak potential shift negatively to 660 mV on glassy carbon electrode modified with the azure Ⅰ films compared with unmodified glassy carbon electrode. The current value had a linear relationship with concentration of NADH over the range of 8.7×10^-5 mol/L to 1.5× 10^-2 mol/L,the detection limit was 3.0×10^-5 mol/L.

关 键 词：NADH 天青Ⅰ 电催化氧化 修饰电极 

分 类 号：O657.15[理学—分析化学]