HPLC法测定羟萘甲酸沙美特罗气雾剂含量  

Determination of content of salmeterol xinafoate in aerosol by HPLC

作　　者：王志宣[1] 杨静文[1] 邓英杰[1] 张晓鹏[1] 陈婉怡 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2006年第10期658-659,675,共3页Journal of Shenyang Pharmaceutical University

摘　　要：目的 建立羟萘甲酸沙美特罗气雾剂药物含量测定的方法。方法 采用HPLC法。色谱柱为Hypersi lC18柱（250mm×4．6mm，5μm），流动相为甲醇-乙腈-水-冰醋酸-三乙胺（体积比为50：20：30：0．07：0．05），流速1．0mL·min^-1，紫外检测波长225mm，室温测定。结果在选择的色谱条件下，理论塔板数以沙美特罗峰计为3520，沙美特罗保留时间为7．5min，辅料不干扰药物的测定。沙美特罗质量浓度在20～120mg·L^-1内，线性关系良好（r=0．9998），平均回收率在99％以上，日内日间RSD均小于2％，重复性小于0．5％。稳定性试验表明，样品溶液稳定性良好。结论 本方法可用于羟萘甲酸沙美特罗气雾剂的质量控制。Objective To study the effects of Buyanghuanwu （traditional Chinese medicines） injection on hemorrheology and experimental thrombus in rats. Methods The acute blood stasis model was made by immersing the rats into ice-water bath and injecting adrenalin hydrochloride（ i. p. ）, and the hemorrheology was measured; the experimental thrombus model was established in rats by artery-vein bypass method in order to observe the effect of Buyanghuanwu injection on the formation of arterial thrombus; the effect of Buyanghuanwu injection on platelet aggregation induced by ADP in rabbits in vitro was studed. Results Buyanghuanwu injection could significantly decrease the viscosities of the whole blood and plasma, inhibit the formation of experimental thrombus in rats, and markedly reduce the ADP- induced platelet aggregation in rabbits in vitro. Conclusions Buyanghuanwu injection has the notable activity of anti-thrombosis, it can reduce the rate of platelet aggregation in rabbits and improve the hemorrheological parameters in acute blood stasis rats.

关 键 词：羟萘甲酸沙美特罗 气雾剂 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]