高温天气血液保存及4℃再保存研究  

A pilot study of red blood cells storage at high temperature

作　　者：骆群[1] 韩玮[1] 毕蔚茹[1] 

机构地区：[1]解放军总医院输血科,北京100853

出　　处：《西北国防医学杂志》2006年第5期365-367,共3页Medical Journal of National Defending Forces in Northwest China

基　　金：军队医药卫生科研基金"十五"指令性课题资助项目(01L045)

摘　　要：目的：研究高温天气条件下血液保存为战时血液保障提供科学依据。方法：采集10名健康献血者全血各200ml，每袋制备成悬浮红细胞后平均分为3袋，分别标记为A组、B组和C组，A组置室外自然温度保存，B组同样条件保存3d后放入4℃冰箱继续保存，C组置4℃冰箱保存，分别监测ATP等保存指标，以C组保存末期（临界）ATP为指示点确定各组保存有效期。结果：悬浮红细胞可在室外26．2℃-36．3℃环境保存4d，若保存3d后再置于4℃条件可继续保存7d。结论：高温天气环境中红细胞可及时有效地进行储运，使伤员得到快速有效的输血救治。Objective：To study the storage method of red blood cell （RBC） at high temperature and provide the scientific evidences for blood preservation and transportation in battle fields. Methods：200 ml of whole blood was obtained from each of the 10 donors and the RBC suspension was prepared. The RBC suspension was divided into 3 groups： A group （stored at room temperature）, B group （stored at room temperature initially for 3 days and at 4℃ later） and C group （stored at 4℃ ）. Several parameters such as ATP were tested daily. The RBC ATP concentration at the end of shelf life in C group served as critical ATP. When RBC ATP decreased to the level of critical ATP, the preservation time was recorded as its shelf life. Results：The RBC suspension could be stored for 4 days at high temperature ranged from 26.2℃ to 36.3℃, and it could continue being stored at 4℃ for 7 days after 3 days of storage at room temperature. Conclusion：The RBC suspension can be stored and transported in its shelf life at high temperature and the wounded can obtain blood transfusion in time.

关 键 词：输血疗法 高温 血液保存 

分 类 号：R457.1[医药卫生—治疗学]