二甲双胍对高糖高脂饮食诱导的糖尿病大鼠脂肪肝治疗作用的研究  

The treatment of metformin in rat model of type 2 diabetes mellitus complicated with fatty liver by high-glucose-high-fat diet

作　　者：王战建[1] 张耀[1] 宋庆芳[1] 庞建华[1] 王振贤[1] 徐焕宇[1] 

机构地区：[1]河北医科大学第三医院内分泌科,河北石家庄050051

出　　处：《中国实用内科杂志：临床前沿版》2006年第10期1603-1605,共3页

基　　金：河北省卫生厅基金资助项目;项目编号:03041

摘　　要：目的探讨二甲双胍对脂肪肝的治疗作用及在改善胰岛素抵抗、调节糖脂代谢中的作用机制。方法2003年2月至2003年7月河北医科大学第三医院通过高糖、高脂饮食诱导并结合小剂量链脲佐菌素注射的方法,建立2型糖尿病大鼠合并脂肪肝的动物模型,再给予二甲双胍625mg/(kg.d)灌胃进行干预治疗10周。18周末检测大鼠血糖、血脂、肝功、肝脏脂质定量、肝脏指数、胰岛素抵抗指数及肝组织学变化,肿瘤坏死因子-α(TNF-α)在肝组织的蛋白表达。结果二甲双胍可以显著降低血糖、血脂、肝脏脂质量,增加胰岛素敏感性,改善肝脏脂肪变程度,降低TNF-α在肝组织的蛋白表达。结论二甲双胍对2型糖尿病合并脂肪肝大鼠的肝脏脂肪变性具有一定的治疗意义。Objective To investigate the functional mechanism of metformin in improving the fatty liver and the treatment of the relative metabolic abnormalities of type 2 diabetes mellitus. Methods The model of type 2 diabetes mellitus complicated with fatty liver was set up by feeding high - glucose - high - fat diet and by injecting STZ. Then, the rats were made an intervention with metformin. At the end of 4 weeks and 8 weeks, the fasting blood glucose, the level of fasting serum insulin, At the end of 18 weeks the fasting blood glucose, the level of fasting serum insulin, ALT, TNF-α and the level of leptin of every group of rats were measured and the expression of TNF-α in the liver was determined. Results Metformin could largely reduce the blood glucose, TG, TC and the fatty content of the liver, improve the state of the insulin resistance and reduce the expression level of the TNF-α in the liver. Conclusion Mefformin can have the treatment functions to the rats with fatty liver in the model of type 2 diabetes mellitus complicated with fatty liver.

关 键 词：非胰岛素依赖型 糖尿病 非酒精性脂肪肝 胰岛素抵抗 二甲双胍 

分 类 号：R5[医药卫生—内科学]