黑曲霉D2-26乳糖酶分离纯化研究  

Study of isolation and purification of lactase from Aspergillus niger D2-26

作　　者：李红飞[1] 于宏伟[1] 韩军[1] 李宁[1] 林杨[1] 贾英民[1] 

机构地区：[1]河北农业大学食品科技学院,河北保定071001

出　　处：《河北农业大学学报》2006年第5期65-68,共4页Journal of Hebei Agricultural University

基　　金：河北省农副产品加工重大科技专项课题"酶工程技术在农产品深加工中的应用研究"资助(03220173D)

摘　　要：为从黑曲霉D2-26发酵液中得到单一的乳糖酶组分,以便研究其酶学性质。试验通过采用过滤、超滤、硫酸铵分级盐析、Sephadex G-25脱盐、DEAE-纤维素-32离子强度梯度层析、Sephadex G-100凝胶过滤、DEAE-纤维素-32 pH梯度层析等方法从发酵液中分离纯化乳糖酶,纯化酶液经PAGE电泳鉴定得到单一蛋白条带。为此建立了一套简单易行的黑曲霉D2-26乳糖酶分离纯化方法。In order to get a single lactase component from the Aspergillus niger D2-26 fermentation liquor to study the enzyme properties, this study was done by means of filtration, ultra-filtration, ammonium sulfate fraction, sephadex G-25 gel filtration, DEAE ion-exchange chromatography, sephadex G-100 gel filtration to isolate and purify the lactase from the fermentation liquor. The purified enzyme identified by PAGE indicated that a single protein band was obtained. The research established a set of simple and feasible ways in the isolation and purification of lactase from Aspergillus niger D2-26 fermentation liquor.

关 键 词：乳糖酶 离子交换层析 分子筛层析 分离纯化 

分 类 号：Q93-3[生物学—微生物学]