日本沼虾卵黄磷蛋白生化性质分析  

Analysis on the characterization of vitellin from the mature ovary of Macrobrachium nipponense

作　　者：穆淑梅[1] 康现江[1] 李彦芹[1] 郭明申[1] 温秀荣[1] 

机构地区：[1]河北大学生命科学学院,河北保定071002

出　　处：《河北农业大学学报》2006年第5期72-75,共4页Journal of Hebei Agricultural University

基　　金：河北大学青年基金(2005Q13);河北省教委项目(97120)资助

摘　　要：采用电泳洗脱法从成熟日本沼虾卵巢中提纯卵黄磷蛋白,并初步分析了其生化性质。聚丙烯酰胺凝胶电泳(PAGE)和SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)结果显示:其相对分子量为458 ku;由3个多肽亚基组成,分子量分别为110 ku,96 ku,89 ku,且三亚基上均有糖辅基的结合;样品缓冲液中β-巯基乙醇的有无并不影响亚基的分离,推测亚基间不存在二硫键。With the PAGE and various staining methods, one form of vitellin （Vn） from the mature ovary of Macrobrachium nipponense was examined. And then, Vn was purified by electric - elution. Using a gradient native PAGE, Vn was found to have a molecular mass of 458 ku. Three subunits （110, 96, and 89 ku） in purified Vn were detected with SDS- PAGE. The three subunits were all glycolpolypep-tides, and nondisulfide bonds were found in the binding of the polypeptide subunits.

关 键 词：日本沼虾 卵黄磷蛋白 生化性质 

分 类 号：Q959.223[生物学—动物学]