1,25(OH)_2D_3对HL-60细胞的抑制增殖和诱导分化作用  

Effects of 1,25-Dihydroxyvitamin D_3 on Inhibition of Proliferation and Induction of Differentiation of HL-60 Cells

作　　者：严舫[1] 程涛[1] 

机构地区：[1]长海医院血液科

出　　处：《第二军医大学学报》1990年第3期213-216,共4页Academic Journal of Second Military Medical University

基　　金：上海市自然科学基金

摘　　要：本文报道1,25(OH)_2D_3对人早幼粒细胞白血病细胞系HL-60的抑制增殖和诱导分化作用。在10^(-9)～10^(-6)mol/L浓度范围内,活细胞计数及克隆形成率显示:1,25(OH)_2D_3对HL-60细胞的抑制增殖作用具有时间和剂量依赖性,10^(-7)mol/L 1,25(OH)_2D_3的克隆形成抑制率为74.82±11.1％。从细胞形态、细胞化学、细胞表面抗原表达以及NBT还原试验等几方面,表明1,25(OH)_2D_3可诱导HL-60细胞向单核细胞部分分化。结合流式细胞分析结果,讨论了细胞增殖与分化的关系。Inhibition of proliferation and induction of differentiation by 1, 25-dihydroxyvitamm D3 of human promyelocytic leukemic cell line (HL-60)are reported. At the concentration range of 109 to 106 mol/ L, 1, 25-dihydroxyvitamin D3 had effect in inhibiting cell proliferation with time-dependent and dose-dependent manner. Strong inhibition of colony cell growth (74.8±11.1%)was observed at 107 mol/L concentration. It was also found that HL-60 cells could be induced into monocytes by 1, 25-dihydroxyvitamin D3at the same concentrations,which were demonstrated by cytochemistry and specific antigen of cell surface and NBT reduction test. In addition, the relationship between proliferation and differentiation is discussed according to the result of flow cytometry.

关 键 词：二羟维生素D3 HL-60细胞 抑制增殖 

分 类 号：R969.4[医药卫生—药理学]