基于二氧化锆/纳米金溶胶凝胶膜固定辣根过氧化物酶的H_2O_2生物传感器的研制  

A Biosensor for Hydrogen Peroxide Based on Horseradish Peroxidase Immobilized in ZrO_2/Au Sol-gel Matrix

作　　者：童忠强[1,2] 袁若[1,2] 柴雅琴[1,2] 陈时洪[1,2] 张凌燕[1,2] 谢轶[1,2] 

机构地区：[1]西南大学化学化工学院,重庆400715 [2]重庆市分析化学重点实验室,重庆400715

出　　处：《西南师范大学学报（自然科学版）》2006年第5期102-106,共5页Journal of Southwest China Normal University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(29705001);教育部优秀青年教师资助项目(教人司200240);重庆市自然科学基金资助项目(CSTC2004BB4149;200513134100);西南大学高新技术培育基金资助项目(XSGX02).

摘　　要：将辣根过氧化物酶(HRP)、二氧化锆溶胶凝胶、纳米金按一定的比例混合,成功制备了以二氧化锆/纳米金溶胶凝胶为载体的性能优良的过氧化氢生物传感.二氧化锆溶胶凝胶具有良好的生物相容性,并提供了良好的微环境保持HPR的生物活性.纳米金与HPR形成静电复合物,有效促进了酶与电极表面的电子传递.该生物传感器性能优良,线性范围为7·0μmol/L至3·9mmol/L,检测下限为4·0μmol/L.最后探讨了pH、工作电位、干扰物质对生物传感器的影响.A highly efficient H2O2 biosensor was fabricated based on the mixture of Horseradish Peroxidase （HRP）, ZrO2 sol-gel and nano-Au immobilized on gold electrode. ZrO2 sol-gel had high biocompatibility feature and provided a suitable microenvironment to keep HPR bioactivity. The presence of nano-Au in matrix can effectively enhance electron transfer between enzyme and electrode since the formation of electrostatic complex of nano-Au-HRP. The biosensor demonstrated excellently electrocatalytical ability for H2O2. The linear range of biosensor for the detection of H2O2 was 7.0 μmol/L to 3.9 mmol/L with a detective limit of 4.0 μmol/L. The performance of biosensor and effects of applied potential, solution pH, temperature and interferences on the biosensor were examined in this paper.

关 键 词：生物传感器 辣根过氧化物酶 纳米金 ZrO2溶胶凝胶 

分 类 号：O657.1[理学—分析化学]