Debaryomyces sp.甘油激酶基本动力学性质研究  

The Kinetic Property of Glycerol Kinase from Debaryomyces sp.

作　　者：谭晓晶[1] 许晓燕[1] 孟尧[1] 武翠玲[1] 赵兴秀[1] 孟延发[1] 

机构地区：[1]四川大学生命科学学院,成都610064

出　　处：《四川大学学报（自然科学版）》2006年第5期1101-1104,共4页Journal of Sichuan University(Natural Science Edition)

摘　　要：通过平板初筛和摇瓶复筛，从105株菌中筛得一株甘油激酶的高产菌株，并研究了其所产甘油激酶的动力学性质．研究结果表明：在MES系统中，甘油激酶有较好的酸碱稳定性，pH稳定范围为5．5～10．5；最适pH和最适温度分别为pH8．0和50℃，该酶催化甘油的米氏常数（Km）为3．5×10^-5mol／L，催化ATP的Km为4．46×10—4mol／L．同时对一些金属离子和有机化合物对酶活性的影响也进行了研究．The strain of Debaryomyces sp. was screened from 105 strains with a rapid metnod, and researcned the kinetic properties of Glycerol Kinase（GK,EC 2.7.1.30） in it. The results showed that： in the system of IVIES, the enzyme was found to have good pH stability from 4.5-8.0, and the optimum pH of enzyme activity was 8.0, the optimum temperature was 50℃. The Km of Glycerol as suhstrate was 3.5 × 10^-5mol/L, and Km value for ATP as substrate was found to be 4.46 × 10^-4mol/L at pH 8.0 and 50℃. The effects of some metal ions and organic compounds on the enzyme activity was studied too.

关 键 词：甘油激酶 德巴利酵母 动力学特性 

分 类 号：Q55[生物学—生物化学]