一株产重楼皂甙内生细菌的分离与鉴定  

Isolation and Identification of an Endopyte of Paris polyphylla var.chinensis Franch

作　　者：张晓洁[1] 查岭生[2] 陈小静[1] 冯定胜[1] 王一丁[1] 

机构地区：[1]四川师范大学生命科学学院 [2]淮北煤碳师范学院生物系,淮北235000

出　　处：《微生物学通报》2006年第5期84-88,共5页Microbiology China

摘　　要：从产自四川彭州的华重楼(Parispolyphyllavar·chinensisFranch)地下块茎中分离得到16株菌。经以薯蓣皂甙元为标准对照的薄层层析分析,表明其中编号为SNUS-1(AY842149)的菌株能分泌重楼皂甙或其类似化合物。16SrDNA序列(约1,500bp)系统发育分析表明:菌株SNUS-1与Pseudomonastolassii(AF255336)和Pseudomonastolassii(D84028)的遗传距离分别为0和0·003,在系统进化树上三者又严格聚为一族,结合形态及生理特征确定其为托氏假单孢菌(Pseudomonastolassii)。Sixteen endophytes were isolated from the hypogenous stems of Paris polyphylla vat. chinensis in Sichuan. Through thin layer chromatography （TLC） with standard comparison of Paris chinensis Franch saponins, the fermentation cultures of strains marked SNUS-1 were identified to contain Paris chinensis Franch saponins or its analogue. A phylogenetic anlysis based on 16S rDNA gene sequences （about 1500bp） shows that the p-distance of the strain SNUS-1 from Pseudomonas tolassii （AF255336） and Pseudomonas tolassii （D84028） are 0 and 0. 003, respectively. These three strains gather into a cluster strictly in the phylogeny tree. Strain SNUS-1 was identified an P. tolassii based on physiological, biochemical and molecular characteristics.

关 键 词：重楼 内生菌 甾体皂甙 16S RDNA 假单孢菌属 

分 类 号：S567.239[农业科学—中草药栽培]