流体力学模型各区域内皮细胞的形态学变化  

Morphological Changes of Endothelial Cells Exposed to Alterative Fluid in a Novel Flow Model

作　　者：王速[1] 赵捷[1] 吉田洋二 陈铁镇 

机构地区：[1]日本国山梨医科大学病理教研室

出　　处：《中国医科大学学报》1996年第6期551-555,共5页Journal of China Medical University

摘　　要：作者设计一个流体力学模型。在此模型的不同区域内不同的液体流动状态下，可产生高低不同的切变应力。应用此模型观察内皮细胞的形态学变化。在模型的斧形流路内，依不同切变应力状态分Ａ、Ｂ、Ｃ、Ｄ四个区域。结果，Ａ区的流速为２４９．８ｍｍ／ｓ，切变应力为７．１９Ｐａ。在最狭窄的Ｂ区，流速和切变应力分别增加到３１６ｍｍ／ｓ和８．８５Ｐａ。在Ｃ、Ｄ区，因液流停滞，流速与切变应力未能测出。在此模型各区域内的内皮细胞的形态和排列方式不同，在Ａ和Ｂ区，细胞呈纺锤形并沿液流方向平行排列。在Ｃ、Ｄ区细胞形态与对照组相似，呈圆或椭圆形。在Ｂ区，Ｆ－肌动蛋白微粒聚集成线状，在胞浆内沿细胞长轴，即液流方向平行排列，在Ｃ、Ｄ区，微粒的分布与对照组类似，主要沿细胞的周边排列。此模型适用于变化的切变应力对内皮细胞形态和功能的影响的研究。A novel flow model was designed and morphological changes of endothelial cells could be seen obserwed in this mode at high or low shear stress regions produced by different flow patterns in areas with accelerated of stagnated flow velocity in a chamber. The axe shaped conduit was designed to give different shear stresses on the cells at A, B, C and D regions. Near the area A, the velocity was 249.8mm/s and shear stress was 7.19 Pa. At B where was the narrowest segment, the velocity and shear stress in creased to 316 mm/s and 8.85 Pa respectively. At the areas C and D, the velocity and shear stress could not be measured because of flow stagnation. The cultured endothelial cells of the swine aorta were exposure to the flow. There were different cell shapes and alignments in different areas of the axe shaped model. The cells at area A and B were spindle and aligned in parallel with the flow direction. At the areas C and D, the cells were round or elliptic as the static controls. At the narrowest area B, F action microfilaments in the endothelial cells were highly assembled band like filaments and celarly visible in their cytoplasms in parallel with long cellular axes with flow direction. At C and D, microfilaments ran along the cell periphery as the controls. These experiments clarify that the novel model can be used to study effects of altering shear stress on morphology and function of endothelial cells.

关 键 词：切变应力 内皮细胞 肌动蛋白 血液动力学 

分 类 号：R318.01[医药卫生—生物医学工程]