切变应力对内皮细胞形态和细胞间连接蛋白的影响  

The Effect of Shear Stress on Morphology and Junction Proteins of Endothelial Cells

作　　者：王速[1] 吉田洋二 陈铁镇 

机构地区：[1]日本国山梨医科大学病理教研室

出　　处：《中国医科大学学报》1996年第6期556-559,共4页Journal of China Medical University

摘　　要：应用流体力学模型观察内皮细胞形态变化和细胞间连接蛋白表达的改变。将培养的猪主动脉内皮细胞置于斧形流路内，使细胞暴露在层流性流动的培养液下。静置培养的对照组内皮细胞极少见紧密连接相关蛋白７Ｈ６的存在。在高切变应力作用７２ｈ后，流路的Ａ和Ｂ区内皮细胞间７Ｈ６蛋白染色性明显增强。但在低切变应力的Ｄ区，此蛋白很少。电镜下观察见到高切变应力作用４８ｈ后，在Ａ和Ｂ区紧密连接构造明显增多。实验结果表明，高切变应力可促进内皮细胞的抗动脉粥样硬化构造—紧密连接的形成。A novel flow chamber was used in order to observe changes of cell morphology and expression of junction proteins of endothelial cells. The cultured endothelial cells of the swine aorta, seeded on polyester sheets, were placed on the bottom of the chamber with axe shaped conduit to be exposed to the laminar and unilateral flow of the culture medium. A tight junction related protein 7H6 was hardly observed in the static cells, but it became visible strongly in areas A and B where were the narrowing and narrowest segments of the conduit, after loading the high shear stress for 72 hours. But at D, the protein was lost at the periphery of the cells. Electron microscopic studies revealed that frequency of tight junctions between endothelial cells in areas A and B was increased by exposure to high shear stress for over than 48 hrs. One of antiatherosclerotic structures of endothelial cells, tight junction, might be developed by laminar unidirectional high shear stress.

关 键 词：内皮细胞 细胞间连接 切变应力 血液动力学 

分 类 号：R318.01[医药卫生—生物医学工程]