金属硫蛋白对大鼠肝脏线粒体氧自由基损伤的保护作用  

PROTECTIVE EFFECT OF METALLOTHIONEIN ON RAT LIVER MITOCHONDRIA DAMAGED BY OXYGEN FREE RADICAL

作　　者：葛淑君[1] 唐朝枢[1] 庞永政[1] 程时[1] 

机构地区：[1]北京医科大学生物物理学系,北京医科大学心血管基础研究所

出　　处：《北京医科大学学报》1996年第6期413-415,共3页Journal of Peking University(Health Sciences)

摘　　要：目的：研究金属硫蛋白（ＭＴ）对氧自由基损伤线粒体的保护作用以探讨其细胞保护机制。方法：采用ＦｅＳＯ４／抗坏血酸自由基发生系统直接损伤线粒体，测定Ｃａ（２＋）－Ｍｇ（２＋）－ＡＴＰａｓｅ活性和Ｃａ（２＋）摄入的变化。结果：ＭＴ能够直接拮抗氧自由基对线粒体Ｃａ（２＋）－Ｍｇ（２＋）－ＡＴＰａｓｅ活性和Ｃａ（２＋）摄入的抑制作用（Ｐ＜０．０１）。结论：ＭＴ对氧自由基损伤线粒体有明显保护作用，ＭＴ对细胞器线粒体的保护作用可能是细胞保护机制之一。Objective:To study the protective effect of metallothionein on rat liver mitochondria dam-aged by oxygen free radical and its mechanism.Methods:On the model of mitochondria damaged by Fe-SO4/ascorbic acid free radical generating system,Ca(2+)/Mg(2+) ATPase activity and Ca(2+) uptake were ob-served and assayed.Results;Metallothionein attenuated decrease of Ca(2+)/Mg(2+) ATPase activity and Ca(2+) uptake induced hy oxygen free radical (P<0.01)in dose-dependent manner.Conclusion;Metal-lothionein could protect mitochondria from oxygen free radical.Mitochondria protection of metalloth-ionein may be involved in the mechanisrn of cell protection of this protein.

关 键 词：金属硫蛋白 药理学 肝线粒体 自由基 

分 类 号：R977.6[医药卫生—药品]