反相高效液相色谱法测定连翘中乌索酸和齐墩果酸的含量  

HPLC Determination of the Contents of Ursolic Acid and Oleanolic Acid in Forsthia suspense(Thunb.)Vahl

作　　者：邹盛勤[1] 余济海[2] 高兴华[3] 陈武[1] 

机构地区：[1]宜春学院生物工程研究所.江西省天然药物活性成分研究重点实验室,江西宜春336000 [2]宜春学院理学院,江西宜春336000 [3]宜春学院生物制药2002级1班,江西宜春336000

出　　处：《时珍国医国药》2006年第10期1872-1873,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家"863"计划重点资助项目(No.2002AA2Z3217)

摘　　要：目的 建立同时测定连翘中乌索酸和齐墩果酸含量的测定方法。方法 采用高效液相色谱（HPLC）法测定其含量。色谱柱为Nova-PakC18（3．9mm×300mm，4μm），流动相为甲醇．水（88：12），流速0．8ml／min，检测波长210nm，柱温25℃。结果 乌索酸和齐墩果酸分别在0．912-4．560μg（r=0．9998）和0．312-1．560μg（r=0．9996）范围内峰面积与进样量呈良好的线性关系；乌索酸和齐墩果酸的平均回收率分别为98．2％（RSD=1．1％）和97．8％（RSD=1．4％）。结论 此法简单、方便、快速、准确，可为中药连翘质量评价和控制提供科学依据。Objective To develop a method for the quantitative analysis of ursolic acid and oleanolic acid in Forsythia suspense simultaneously. Methods HPLC was carried out on the Nova-Pak CIs column （3.9 mm × 300 mm, 4 μm） at 25℃ using MeOH- H20（88： 12） as mobile phase with a flow rate of 0.8 μl and detection wavelength was set at 210 nm . Results There was good linear relationship between the peak area and the sample content injected at the ranges of 0. 912-4. 560μg （r = 0. 999 8 ）and 0. 312 -1.560 μg（r = 0. 9996）for ursolic acid and oleanolic acid respectively . The average recovery rate of ursolic acid and oleanolic acid were 98.2 % （RSD 1.1% ） and 97.8 % （ RSD 1.4 % ）, respectively. Conclusion It＇s a simple, convenient, quick and accurate method, and can also be used scientifically in the quality estimation and control of Chinese traditional medicine Forsythia Suspense.

关 键 词：连翘 乌索酸 齐墩果酸 高效液相色谱 

分 类 号：R284.2[医药卫生—中药学]