文心兰规模化组培育苗关键技术研究  

Key Technology Study on Oncidium Industrial Propagation by Tissue Culture

作　　者：欧阳彤[1] 陈胜[1] 汪凤珍[1] 

机构地区：[1]中国林业科学研究院亚热带林业研究所,浙江富阳311400

出　　处：《林业科学研究》2006年第5期606-611,共6页Forest Research

基　　金：中国林科院亚热带林业研究所创新基金(2004-C-02)

摘　　要：通过基本培养基（改良MS、改良KC、V＆W及自制ZW系列）、植物激素（6-BA、KT、NAA、IBA）、培养条件（香蕉泥、蛋白胨、酵母提取物、糖、活性炭）等关键因子对文心兰组培各阶段影响的试验，探索了文心兰规模化组培育苗的关键技术。结果表明：（1）当6-BA0．1～2mg·L^-1，NAA0．1～1．0mg·L^-1时，可诱导拟原球茎形成与增殖；6-BA0．05～0．1mg·L^-1或附加NAA0．1mg·L^-1就可满足拟原球茎的分化；6-BA0．01mg·L^-1协同NAA或IBA0．1mg·L^-1处理，诱导生根快而粗壮；（2）添加活性炭1000mg·L^-1和维生素C30mg·L^-1对减轻外植体褐化是必要的；添加500～1000mg·L^-1蛋白胨或酵母提取物可促进诱导拟原球茎；香蕉泥100g·L^-1有助于拟原球茎分化和瓶苗健化；（3）MS高盐类培养基在每个培养阶段都有较高的致畸率，自制中盐ZW培养基，N：P：K为3：1：2．9，文心兰在其中生长健壮；（4）文心兰在无糖培养基中自养生长基本正常。The key technology of Oncidium＇ s industrial propagation were studied by in situ investigating the effects of the key factors such as minimal medium （improved MS, improved KC, V＆W and self-prepared ZW series,etc. ）, phytohormone （6-BA, NAA, IBA, KT）, additive （vitamin C, peptone, mashed banana, active carbon,sugar, etc. ）, on the mass b that ： （ 1 ） The protocorm like reeding in every stage during Oncidium＇s industrial body （PLB） could be induce and increase as long propagation. The results revealed as the concentration of 6-BA between 0.1- 2 mg·L^-1 and NAA 0.1 - 1.0 mg·L^-1. At the revulsive initial stage a concentration of 6-BA 2 mg·L^-1 and with NAA 0.2 mg·L^-1 was helpful to increase the propagation basic number of protocorm like body. The multiply and differentiation of PLB could take place as long as 6-BA 0.05 - 0.1 mg· L^-1 or addition of NAA 0.1 mg·L^- 1 .The root emerging could be accelerated and the roots become stronger cooperation of addition of low concentration 6-BA （0.01 -0.1 mg·L^-1 ） together with NAA or IBA（0.1 mg·L^-1 ）. （2）The low concentration of auxin （0.01 - 0.1 mg·L^-1 ） and the sort of low concentration of mineral medium （ such as V＆W, KC, etc. ） seemed to benefit the decrease of the malformation during PLB differentiation and seedling growth. （3）In order to reduce the explant browning addition of active carbon 1 000 mg·L^-1 and vitamin C 30 mg·L^-1 was necessary. Adding peptone 500 -1 000 mg·L^-1 or drawn substance of yeast seemed to promote the induction of PLB. Mashed banana 100 g ·L^-1 might benefit the PLB differentiation and improve the quality of test-tube plantlet. （4） Minimal mediums had notable effects on every stage of Oncidium tissue culture. The self-prepared ZW mediums with middle concentration of salt were more suitable to Oncidium propagation by tissue culture. （5）Oncidium was autotrophic without sugar in the medium.

关 键 词：文心兰 组织培养 规模化育苗 关键技术 

分 类 号：S722.3[农业科学—林木遗传育种]