黄瓜霜霉病菌烯肟菌酯敏感基线的建立及其室内抗药性风险评估  

Baseline-sensitivity and assessment of resistance risk of Pseudoperonospora cubensis to enostrobilurin

作　　者：王岩[1] 冯明鸣[1] 朱书生[1] 刘西莉[1] 

机构地区：[1]中国农业大学农学与生物技术学院,北京100094

出　　处：《中国农业大学学报》2006年第5期25-29,共5页Journal of China Agricultural University

基　　金：国家科技支撑计划粮食丰产科技工程项目(2004BA520A15)

摘　　要：为研究黄瓜霜霉病菌对烯肟菌酯的敏感性及室内抗药性风险，采用叶碟保湿法测定了52株黄瓜霜霉病菌对烯肟菌酯的敏感性。结果表明52个菌株的EC50（抑制中浓度）差异较小，平均EC50为（0．0101±0．0031）μg／mL。黄瓜霜霉病菌对烯肟菌酯的敏感性频率分布呈连续单峰曲线，接近正态分布，没有出现敏感性下降的抗药性群体，这些病菌均为烯肟菌酯野生敏感菌株，因此可以采用其平均EC如作为黄瓜霜霉病菌对烯肟菌酯的敏感基线。室内药剂驯化和紫外线诱变试验，获得了10株黄瓜霜霉病菌对烯肟菌酯不同抗性水平的突变体，其EC50为1～5μg／mL，抗性指数为80～500倍，抗药性稳定，致病力、适合度、竞争力与亲本菌株相比没有明显差异，或优于亲本菌株。室内抗药性风险评估表明，黄瓜霜霉病菌对烯肟菌酯具有较高的抗药性风险，预示田间自然情况下抗药性菌株可能形成优势菌群而导致药剂防治失败的潜在风险。The baseline-sensitivity of the obligate parasite pathogen Pseudoperonospora cubensis to enostrobilurin was studied. Determined by detached leaves in vitro, the mean EC50 values was（0.010 1 ± 0.003 1） μg/mL, and there was no resistant subpopulation among strains. Hence, this baseline sensitivity was suitable for field resistance monitoring. Ten highly enostrobilurin-resistant mutants were selected with resistance factors of 80 - 500 folds. The pathogenicity, fitness and competition of the enostrobilurin-resistant mutants were not significantly different from those of their parental strains. The level of resistance of enostrobilurin-resistant mutants was stable after 10 disease cycles on healthy leaves without enostrobilurin. Laboratory studies showed that resistance of P. cubensis to enostrobilurin was a high risk. Therefore, the epidemic development of the resistant population of P. cubensis would be possible in the field.

关 键 词：黄瓜霜霉病菌 烯肟菌酯 敏感基线 抗药性风险 

分 类 号：S481.4[农业科学—农药学] S436.421.11[农业科学—植物保护]