棉花γ射线诱变后代的SSR标记遗传多样性  

Genetic Diversity of the Progeny of Cotton (Gossypium hirsutum L.)Irradiated by γ Ray with SSR Markers

作　　者：孙君灵[1] 杜雄明[1] 孙其信[2] 周忠丽[1] 潘兆娥[1] 庞保印[1] 

机构地区：[1]中国农业科学院棉花研究所,安阳455004 [2]中国农业大学植物遗传育种系,北京100094

出　　处：《中国农业科学》2006年第10期1967-1976,共10页Scientia Agricultura Sinica

基　　金：国家"863"计划项目(2004AA227140);"十五"国家科技攻关项目(2004BA525B05)资助

摘　　要：【目的】了解γ射线诱变对棉花后代SSR标记遗传多样性的影响。【方法】利用39个多态性SSR标记分析了3个棉花品种辐射诱变后代M5材料(共74份)的遗传多样性。【结果】3个品种诱变后代的成对相似系数变幅分别为0.6129～0.9848、0.6212～1.000、0.4857～0.9905,其中Arcot-1和Su9108两个品种诱变后代的成对相似系数大于或等于8.000的比例达到60.0%以上,J11诱变后代的成对相似系数主要集中在低相似性区域,其对成相似系数小于7.000的比例达62.5%。3个品种诱变后代材料与其未辐射处理对照的遗传相似系数变幅分别为0.6515～0.9697、0.6970～1.0000、0.5524～0.8095。【结论】辐射可创造丰富的遗传变异,拓宽原品种的遗传基础。辐射对不同棉花品种的诱变效果存在差异。[Objective] The objective of this study is to understand the effect of γ-ray irradiation on cotton. [Method] The genetic diversity of 74 mutagenic progenies M5 in 3 cotton varieties, Arcot-1, Su9108 and J11, irradiated by γ-ray were analyzed with 39 polymorphism SSR primer pairs. [ Result] The variance range of mutagenic progeny＇s （Ms） pair similarity coefficient of 3 cotton varieties were 0.6129 - 0.9848, 0.6212 - 1.000, 0.4857 - 0.9905 respectively. The pair similarity coefficient that was higher or equal to 8.000 was more than 60.0% for the mutagenic progenies M5 of two varieties （Arcot-1 and Su9108）. The pair similarity coefficient less than 7.000 was 62.5% for the mutagenic progenies M5 of J 11. The variance ranges of genetic similarity coefficient of mutagenic progeny M5 SSR markers of 3 cotton varieties and their original CK were 0.6515 - 0.9697, 0.6970 - 1.000, 0.5524 - 08095 respectively. [ Conclusion] The analysis of molecular markers indicated that abundant genetic variations can result and genetic bases in original varieties can be broadened from the γ-ray radiation. The study also clarified that the radiation of different cotton varieties resulted in the different variation.

关 键 词：棉花 SSR标记 辐射 遗传多样性 

分 类 号：S562[农业科学—作物学]