脾气虚胃溃疡证病结合模型甲状腺组织乳酸脱氢酶和琥珀酸脱氢酶变化研究  

To Probe the Changes of Enzymes in the Model of Splennic Astbenia Complicating Gastric Ulcer.

作　　者：陆佰荣 杜鹃[2] 王秀琴[2] 孟庆峰[2] 廉洁[3] 张海艳[3] 

机构地区：[1]北京市大兴区医学教育培训中心,102612 [2]首都医科大学组胚教研室,100054 [3]齐齐哈尔医学院组胚教研室,161042

出　　处：《医学研究杂志》2006年第10期97-98,共2页Journal of Medical Research

摘　　要：目的探讨脾气虚胃溃疡证病结合模型甲状腺组织代谢酶的改变,进一步揭示脾气虚胃溃疡和甲状腺之间的关系。方法本实验是将大鼠制成脾气虚胃溃疡证病结合模型,对其进行加味四君子汤治疗,在溃疡最典型的第4天和溃疡基本愈合的第14天取材,进而将实验动物分第4天、第14天的脾溃组、治疗组、自然恢复组、盐水组各4组,对各组甲状腺组织的酶进行酶组化染色,通过图像分析其光密度值并经统计学处理。结果与盐水组比较,脾溃组酶的活性降低,治疗组明显恢复,自然恢复组接近脾溃组。结论脾气虚胃溃疡证病结合模型甲状腺出现了损伤,加味四君子汤对损伤的甲状腺有治疗作用。Objective To probe the changes of enzymes in the model of splennic asthenia complicating gastric ulcer,and reveal the relation between thyroid gland and splennic asthenia gastric ulcer. Methods Building model of splennic asthenia complicating gastric ulcer in rats, treatment them in plus sijunzi decoction,selecting rats with typical gastric ulcer four days after and rats with the ulcer healing basically fourteen days after building models, then the rats were divided into four groups, the model group, the treatment group, spontaneous recovery group and the control group. With histochemical stain,the OD values of enzymes were analyzed with image analysis, and were analyzed statistically. Results the activities of lactate dehydrogenase （LDH）, succinate dehydrogenase （SDH）, decreased. Compare the treatment group recovered. Conclusions In the model of splennic asthenia complicating gastric ulcer,some injury can occur on thyroid gland. plus sijunzi decoction has some treating action.

关 键 词：脾气虚 胃溃疡 甲状腺 酶组织化学染色 乳酸脱氢酶 琥珀酸脱氢酶 

分 类 号：R256.3[医药卫生—中医内科学]