甲基红共振瑞利散射法测定肝素钠  

Resonance rayleigh scattering method for the determinaion of heparin with methyl red

作　　者：郭小群[1] 

机构地区：[1]四川理工学院邓关校区化学系,四川自贡643033

出　　处：《化学研究与应用》2006年第10期1242-1244,共3页Chemical Research and Application

基　　金：四川省教育厅重点专项(2005D007)资助项目;四川理工学院教改项目(JG-0619)资助

摘　　要：We Investigated the spectra of Resonance Rayleigh scattering(RRS)for methyl red-heparin system,the spectra characteristics,the primary efiect factors and the optimum condition of the reaction.The resuIts show that the method was based on the discoloration reaction between heparin and methyl red dyes in the pH 6.6 Britton-Robinson solution.The linear range was 0.006μg/mL-4.00μg/mL.The reductiOn in absorbance is directly proportional to the concentration of heparin.and the efiect of surfactants and coexistion substance was investigated.The method has been applied to determine the potency of heparin sodium injection and the heparin sodium in human serum with satisfactory results.We Investigated the spectra of Resonance Rayleigh scattering （RRS）for methyl red-heparin system, the spectra characteristics,the primary effect factors and the optimum condition of the reaction. The results show that the method was based on the discoloration reaction between heparin and methyl red dyes in the pH 6.6 Britton-Robinson solution. The linear range was 0. 006μg/ mL-4. 00μg/mL. The reduction in absorbance is directly proportional to the concentration of heparin, and the effect of surfactants and coexistion substance was investigated. The method has been applied to determine the potency of heparin sodium injection and the heparin sodium in human serum with satisfactory results.

关 键 词：共振瑞利散射 肝素钠 甲基红 

分 类 号：O675.39[理学—化学]