HPLC法测定大鼠血浆中抗肿瘤化合物SYUIQ-5的浓度  

Determination the plasma concentration of SYUIQ-5,a novel antitumor compound,by HPLC in rat

作　　者：苏启表[1] 和凡[1] 卢宇靖[1] 古练权[1] 黄民[1] 

机构地区：[1]中山大学药学院临床药理研究所,广东广州510080

出　　处：《中国临床药理学杂志》2006年第4期295-298,共4页The Chinese Journal of Clinical Pharmacology

基　　金：广州市重大项目一创新药物药理毒理评价研究技术平台资助项目(2003Z2-E4071)

摘　　要：目的建立一种灵敏度较高的高效液相色谱法,测定大鼠血浆中抗肿瘤化合物SYUIQ-5的浓度.方法流动相用35 mmol·L^-1磷酸氢二钾缓冲液（pH8.0）-甲醇-三乙胺（30：70：0.05）,血浆样本用乙醚萃取,紫外检测波长为278nm.结果线性范围为30～1 500 μg·L^-1,线性关系良好,最低检测限为20μg·L^-1,日内RSD≤5.2%,日间RSD≤3.8%;提取回收率为72.1%～80.5%（n=5）,相对回收率为96.8%～105.1%.结论本方法灵敏度高,重现性好,精密度和准确性好,适于SYUIQ-5的药代动力学研究.Objective To determinate the plasma concentration SYUIQ- 5 by establishing HPLC method, a novel anti -tumor compund in rat. Methods Plasma samples were cleaned up by extraction with diethyl ether. The chromatogram was monitored by UV detection at a wavelength of 278 urn. The mobile phase consisted of 35 mmol·L^-1 dipotassium hydrogen phosphate （pH 8.0） - methanol - triethylamine（30：70：0.05）. Results A linear range was 30 - 1 500μg· L^-1, the lower limit of detection was 20μg· L^- 1. The intra - and inter -nm precision were lower than 5.2% and 3.8% （n =5）, respectively. The extraction recovery was 72. 1% - 80.5% （n = 5 ） and the method recovery was 96.8% - 105.1%. Conclusion The me - thod is shown to be accurate and repetitive, simple to operate, suitable for clinical pharmacokinetic study .

关 键 词：高效液相色谱法 血药浓度 药代动力学 

分 类 号：R969.1[医药卫生—药理学] R979.1[医药卫生—药学]