知母总皂苷对大鼠脑皮质乙酰胆碱酯酶的抑制作用  

Effect of Saponins from Anemarrhena asphodeloides Bge on Activity of Acetylcholinesterase in Cortices of Rats

作　　者：欧阳石[1] 孙莉莎[1] 徐江平[1] 

机构地区：[1]南方医科大学药学院药理学系,广州510515

出　　处：《中国药学杂志》2006年第19期1472-1474,共3页Chinese Pharmaceutical Journal

基　　金：国家863计划新药博士基金资助项目(2003AA2Z3535)

摘　　要：目的研究知母总皂苷(SAaB)在体内外对大鼠脑皮质中乙酰胆碱酯酶(AchE)的影响。方法用东莨菪碱致大鼠记忆获得性障碍模型,观察知母总皂苷对模型大鼠脑皮质匀浆AchE的影响;用比色法测定知母总皂苷溶液和含药血清对大鼠脑皮质匀浆AchE的体外抑制作用。结果知母总皂苷可显著降低东莨菪碱模型大鼠脑皮质内AchE活力(P<0·01);知母总皂苷对大鼠脑皮质匀浆AchE活力没有直接的抑制作用(P>0·05),而含药血清可明显抑制大鼠脑皮质匀浆AchE的活力(P<0·01)。结论知母总皂苷在大鼠体内经吸收转化后可抑制大鼠脑皮质AchE活性。OBJECTIVE To observe the effect of Saponins from Anemarrhena asphodeloides Bge （SAaB） on the activity of acetyl- cholinesterase（AchE） in cortices of rats.METHODS The dysmnesia model of acquired learning induced by scopolamine was used. The activity of Ache in cortices of model animal was determined, Serum containing SAaB was prepared by serum pharmacological method. Brain cortices homogenate was treated with the serum and the SAaB solution respectively, and the influence of SAaB on Ache activity was determined by Ache test kit, RESULTS SAaB antagonized the increase of Ache induced by scopolamine （ P 〈 0.01 ）. The serum containing SAaB was shown to inhibit AchE（ P 〈 0.01 ） in vitro while there was no SAaB effect on AchE（ P 〉 0.01 ）. CONCLUSION SAaB can inhibit the activity of Ache after the absorption and transformation in vivo.

关 键 词：知母总皂苷 乙酰胆碱酯酶 

分 类 号：R965[医药卫生—药理学]