RP-HPLC测定西洋生晒参和西洋红参中人参皂苷含量  

Determination of Ginsengnosides in Dry Radix quinguefolii and Red Radix quinguefolii By RP-HPLC

作　　者：郑友兰[1] 杨春花[1] 鲍建才[1] 肖杨[1] 刘刚[1] 

机构地区：[1]吉林农业大学中药材学院,长春130118

出　　处：《中国药学杂志》2006年第19期1494-1496,共3页Chinese Pharmaceutical Journal

基　　金：国家科技部资助项目(99-929-01-05)

摘　　要：目的 RP-HPLC测定西洋生晒参和西洋红参中人参皂苷含量。方法 利用Agilent 1100 Series高效液相色谱仪进行测定。色谱柱为德国Nudeosil—C18（4．6mm×150mm，5μm）；流动相为乙腈-水梯度洗脱，流速为1．5mL·min^-1；检测波长203nm。结果 西洋生晒参和西洋红参均含有较高的人参皂苷Rb1，Re，与西洋生晒参比较，西洋红参在保留时间27～28min处的Re，51min的Rb1及64min处的一未知化合物的含量均有明显变化，此点在以往的西洋红参研究中鲜见报道。结论 本方法简便可靠、快速、重现性好，适用于西洋生晒参和西洋红参中人参皂苷含量测定，为进一步研究西洋红参的成分及其药理作用提供了参考数据。OBJECTIVE To develop a HPLC method for the determination of Ginsenosides in Dry Panax quinguefolius L . and Red Panax quinguefolius L.METHODS Agilent 1100 Series instrument was used. The separation was performed on a Nudeusil - C19（4.6mm× 150mm,5 μm） with gradient elution. The mobile phase consisted of acetonitrile-H2O（48：52） ; the flow rate of 1.5 mL·min^-1; the detection wavelength was at 203 nm.RESULTS The contents of ginsenosides-Rb1 and ginsenosides Re were both high in Dry Pannx quinguefolius L.and Red Panax quinguefolius .Compared with Dry Panax quinguefolius L,the contents of ginsenosides Re with the retention time of 27 - 28 min, ginsenosides-Rb1 with the retention time of 51 min and an unknown compound with the retention time of 64 min were changed obviously. CONCLUSION This method is simple, convienent, rapid and suitable for the determination of Giasenosides in Dry Panax quinguefoliu L. sand Red Panax quinguefolius L..

关 键 词：高效液相色谱法 西洋生晒参 西洋红参 人参皂苷 

分 类 号：R284[医药卫生—中药学]