利用分子印迹技术分离葛根异黄酮  

Separation of isoflavones from Radix Pueraria by molecular imprinting technology

作　　者：程绍玲[1] 杨迎花[1] 

机构地区：[1]天津科技大学理学院,天津300222

出　　处：《中成药》2006年第10期1484-1488,共5页Chinese Traditional Patent Medicine

基　　金：天津科技大学自然科学基金资助

摘　　要：目的:研究M IP对葛根素的吸附机理并进行Scatchard分析。方法:以葛根素为模板分子,丙烯酰胺为单体,二甲基丙烯酸乙二醇酯(EDMA)为交联剂,制备了葛根素分子印迹聚合物(M IP)。结果:表明M IP对葛根素有两类结合位点。用葛根素M IP对葛根提取物进行了分离。结论:HPLC分析结果表明分离产物中主要含有葛根素及其它两种异黄酮成分。AIM： To investigate the adsorption mechanism of pueraria on the molecular imprinting polywer （MIP） and Scatchard analysis was carried out. METHODS： Pueraria MIP was synthesized using pueraria as the template, acrylamide as the functional monomer and ethylene glycol dimethaerylate （EDMA） as the crosslinker. RESULTS： The result showed that at least two classes of binding sites were formed in the imprinted polymer. Extracting compounds from Radix Pueraria was separated by MIP. CONCLUSION ： The component of the products is analyzed by HPLC. The result shows that the final products contain pueraria and the other two kinds of isoflavone.

关 键 词：分子印迹聚合物 葛根 葛根素 黄酮 

分 类 号：R284.1[医药卫生—中药学]