小檗碱对D-半乳糖诱导糖基化大鼠脑损害的干预作用  

Improvement effects of berberine on glycated brain damages in rats induced by D-galactose

作　　者：吕俊华[1] 张世平[1] 郑浩[1] 

机构地区：[1]暨南大学药学院药理教研室,广东广州510632

出　　处：《中成药》2006年第10期1466-1469,共4页Chinese Traditional Patent Medicine

摘　　要：目的:研究小檗碱(Berberine)对D-半乳糖诱导的糖基化模型大鼠并发脑损害的干预作用。方法:D-半乳糖(150mg/kg.d,ip)连续给药8周,诱导糖基化模型大鼠,并于第3周开始给予小檗碱高(300 mg/kg)、中(150 mg/kg)、低(75mg/kg)剂量处理6周。测定红细胞醛糖还原酶活性、糖化血红蛋白、血清果糖胺和晚期糖基化终末产物(advanced glyca-tion end-products,AGEs)含量及脑组织中AGEs含量、脑神经细胞内钙离子水平,并以透射电镜观察脑内海马神经原线粒体的变化。结果:小檗碱高、中剂量明显降低模型组大鼠红细胞醛糖还原酶活性,抑制糖化产物的形成(P<0.01),降低脑组织中AGEs及脑神经细胞内钙的含量(P<0.05,P<0.01),保护海马神经原线粒体结构的完整性。结论:小檗碱具有抑制D-半乳糖诱导的蛋白糖基化反应,并对糖基化状态并发的脑神经细胞损害具有保护作用。AIM： To observe the improvement effects of berberine on glycated brain damages in model rats induced by D-galactose. METHODS： The model rats of protein glycation were induced by intraperitoneal administration of D-galactose （150 mg/kg·d） for 8 weeks,and all rats were treated with berberine （high dose 300 mg/ kg, middle dose 150 mg/kg, low dose 75 mg/kg） for 6 weeks. The activity of aldose reductase in red blood cells, the amount of glycated products （ fructosamine in serum, glycohaemoglobin, advanced gtycation end-products）, and the content of AGEs in brain tissue, calcium ion in brain cells were measured. Moreover, mitochondria in brain hippocampus cells were observed under electronic microscope. RESULTS： High dose and middle dose of berberine could decrease the activity of aldose reductase in red blood cells （P〈0.01 ） ,and inhibit the formation of glycation products significantly in model rats induced by D-galactose （P〈0.01 ）. Also,berberine could decrease the content of AGEs in brain and the level of calcium ions in brain cells （ P〈0.05, P〈0.01 ）, and also decrease lesions deglycated brain damages through inhibiting the glycation reaction in rats induced by D-galactose.

关 键 词：小檗碱 D-半乳糖 糖基化 钙离子 线粒体 

分 类 号：R392.8[医药卫生—免疫学]