鹅卵泡颗粒细胞凋亡及其与生殖激素间的关系  

Apoptosis of Goose Granulosa Cells and Its Relationship between Reproductive Hormones

作　　者：陈秀萍[1] 姜勋平[1] 丁家桐[2] 

机构地区：[1]华中农业大学动物科技学院,武汉430070 [2]扬州大学动物科学与技术学院,扬州225009

出　　处：《畜牧兽医学报》2006年第10期982-986,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金项目(30300253);中国博士后科学基金项目(2003033469)

摘　　要：采用正处于产蛋期的扬州鹅10只，取其卵泡分离颗粒层细胞，用70％乙醇固定后，应用流式细胞术分析颗粒细胞凋亡情况。同时，用连续采血的方法，每3h采血一次，分离血浆，用放射免疫分析法测定血浆促卵泡素（FSH）、促黄体素（LH）、雌激素（Ez）水平，用以分析激素水平与凋亡间的关系。结果表明：（1）无论是健康卵泡还是闭锁卵泡，颗粒细胞90％以上均处于G1期，2％～6％处于G2期，S期比例最小；（2）闭锁卵泡颗粒细胞凋亡率极显著高于健康卵泡（P〈0．01）；（3）随着健康卵泡直径的增加，颗粒细胞的凋亡呈降低趋势；（4）健康组鹅的FSH、E2水平要高于闭锁组，而LH水平要低于闭锁组。Ten laying-egg-yangzhou-gooses were sacrificed to obtain their follicles, from which granulosa cells were collected. After the granulosa cells were separated, they were fixed by 70% ethanol, and the apoptotic cell death was evaluated by flow cytometry. Meanwhile, the plasma samples were collected to evaluate the level of FSH,LH and E2 by R1A methods. The relationships between hormone contents and cell apoptosis rate were calculated. The results showed that： （1） Whether the follicle was healthy or atresic, the percentage of G1 phase of granulose cells was more than 90 %, about 2 %--6 % granulosa cells were in G2 phase, and the percentage of S phase was the lowest; （2） The apoptosis rate of granulosa cells of atresia follicles was extremely significant higher than that of healthy follicles（P〈0.01） ; （3） With the incensement of follicular diameter, the apoptosls rate of healthy follicles decreased correspondingly; （4） The level of FSH,E2 of healthy group were higher than that of apoptotic group, while the level of LH was on the other hand.

关 键 词：扬州鹅 细胞凋亡 颗粒细胞 生殖激素 

分 类 号：S835.2[农业科学—畜牧学]