组培条件对油樟试管苗玻璃化的影响  

Study on the Vitrification of Cinnamomum longepaniculatum (Gamble) N.Chao in Tissue Culture

作　　者：魏琴[1] 周黎军[2] 宣朴[3] 岳春芳[3] 周锦霞[2] 张新申[1] 

机构地区：[1]四川大学轻纺与食品学院,四川成都610065 [2]宜宾学院生物工程系,四川宜宾644007 [3]四川省农科院生物技术与核技术研究所,四川成都610066

出　　处：《四川师范大学学报（自然科学版）》2006年第5期606-608,共3页Journal of Sichuan Normal University（Natural Science）

基　　金：四川省教育厅青年基金;四川省科技厅应用基础研究基金资助项目

摘　　要：研究了外植体年龄、处理方式对油樟试管苗玻璃化的影响及封口膜的透气性和培养基中不同浓度的蔗糖、琼脂、细胞分裂素(BA)浓度等因素对油樟试管苗玻璃化的影响.结果表明,以当年生带芽茎段为外植体玻璃化苗少,污染少;玻璃化苗的数量随着糖分及琼脂浓度的降低、BA浓度的增加而加重;封口膜的透气性与油樟苗的玻璃化关系不大.通过在培养基中适当调整蔗糖、琼脂、BA的浓度,可有效地控制油樟玻璃化苗数.The effects of explant age and treatment, mixing sugar concentration, agar concentration, BA concentration in culture medium and gas permeability on the vitrification of Cinnamomum longepaniculatum （Gamble）N. Chao in tissue culture were investigated. When current growth stems with buds were used as explants, the numbers of vitrified or polluted shoots were less than that of the others, The numbers of vitrified plantlet increase with decreasing of sugar and agar concentration as well as increasing of BA concentration. The gas permeability of seal membrane almost has no relation to vitrification. The numbers of vitrified plantlet of Cinnamomum longepaniculatum （Gamble）N. Chao should be controlled by manipulating the concentrations of sugar, agar and BA in the culture medium.

关 键 词：油樟 玻璃化现象 组织培养 

分 类 号：Q813[生物学—生物工程]