溶血磷脂酸对胚胎干细胞分化心肌细胞L型钙通道电流的影响  

Effect of lysophsophatidic acid on L-type calcium channel current in embryonic stem cell-derived cardiomyocytes

作　　者：罗浩[1] 江洪[1] 曾彬[1] 蔡军[1] 王腾[1] 

机构地区：[1]武汉大学人民医院心内科,湖北武汉430060

出　　处：《中国心脏起搏与心电生理杂志》2006年第5期431-433,共3页Chinese Journal of Cardiac Pacing and Electrophysiology

摘　　要：目的诱导小鼠胚胎干(ES)细胞向心肌细胞分化并观察溶血磷脂酸(LPA)对分化心肌细胞L型钙通道电流(ICa-L)的影响。方法采用悬滴培养法诱导小鼠ES细胞向心肌细胞分化,逆转录-聚合酶链式反应及免疫荧光检测心肌细胞特异性标志物,全细胞膜片钳记录LPA0.1,1.0和10μmol/L对小鼠ES细胞分化心肌细胞ICa-L的影响。结果小鼠ES细胞成功向心肌细胞分化。0.1,1.0和10.0μmol/LLPA使分化心肌细胞ICa-L峰电流密度分别由用药前-6.8±0.7pA/pF增加到-8.9±1.2,-12.6±2.9和-16.6±3.5pA/pF(P<0.01或P<0.05)。结论LPA呈浓度依赖性促进小鼠ES细胞分化的心肌细胞ICa-L。Objective To differentiate murine embryonic stem（ES） cells into cardiomyocytes and investigate the effects of lysophsophatidic acid （LPA） on ICa-L in ES cell-derived cardiomyocytes. Methods Differentiated ES cells into cardiomyocyte-like cells. Immunocytochemistry staining and reverse transcription-polymerase chain reaction （RT-PCR） indicated that the cells derived from ES cells were cardiomyocytes based on the presence of cardiac specific molecular marker. Gathered ES cell-derived cardiomyocytes and recorded the ICa-L currents in a whole cell clamp configuration. Results Murine ES cells differentiated into cardiomyocytes successfully. LPA （0.1,1.0 and 10.0μmol/L） increased the ICa-L from -6.8 ±0.7 pA/pFto -8.9±1.2, -12.6±2.9, -16.6±3.5pA/pF（P〈0.05 or P〈0.01）. Conclusion LPA canincrease the ICa-L in ES cell-derived cardiomyocytes in a concentration-dependent manner. [ Chinese Journal of Cardiac Pacing and Electrophysiology, 2006,20 （ 5 ） ：431 - 433 ]

关 键 词：心血管病学 溶血磷脂酸 胚胎干细胞 分化 心肌细胞 L型钙通道 

分 类 号：R331.38[医药卫生—人体生理学]