三七不定根的离体诱导与培养  

Adventitious root induction and in vitro culture of Panax notoginseng

作　　者：高先富[1] 徐朝晖[1] 刘佳健[1] 马丽萍[1] 尹龙萍[1] 贾伟[1] 

机构地区：[1]上海交通大学药学院,上海200240

出　　处：《中国中药杂志》2006年第18期1485-1488,共4页China Journal of Chinese Materia Medica

摘　　要：目的:探讨三七不定根的诱导与体外培养方法。方法:通过从大田栽培三七外植体、再生苗外植体和愈伤组织诱导3种途径获得不定根,考察2,4-二氯苯氧乙酸(2,4-D)、吲哚-3-丁酸(IBA)、萘乙酸(NAA)等植物生长物质对不定根诱导的影响,比较4种不定根与其母体分离方式对其体外液体培养的影响。结果:3种方法均获得不定根,幼嫩花蕾愈伤组织是诱导不定根的理想材料;IBA诱导不定根的能力最强;采用将不定根连其母体组织一起在液体培养系统中连续培养后再分离的方法建立了液体培养体系。结论:成功诱导三七不定根并建立了液体培养体系。Objective： To investigate the induction and culture of adventitious root of Panax notoginseng. Method： Three ways, induction from the explants of three-year-old P. notog/nseng. The explants of regenerated shoots and calluses, were used to induce adventitious roots. The effects of 2, 4-dichlorophenoxyacetic acid, indole-3-butyric acid and naphthylacetic acid on adventitious root induction were investigated respectively. The effects of four modes of separating adventitious roots from the parent tissues on culture in vitro were compared. Result： Adventitious roots were successfully induced by three methods, of which the young flower bud callus was the best material for the induction of adventitious root. Indole-3-butyric acid possessed the strongest potency for induction. The liquid culture, system was established by continuous culture of adventitious roots together with their parent tissues before separated. Conclusion： The acquisition and culture in vitro in liquid culture system of adventitious roots of P. notoginseng lay a foundation for the next investigation.

关 键 词：三七 不定根 诱导 

分 类 号：S567.236[农业科学—中草药栽培]