不同染色法检测豚鼠皮肤黑色素的比较研究  

Comparative Study on Different Stain Methods for Melanin in Guinea Pig Skin

作　　者：董妙珠[1] 肖萍[1] 洪新宇[1] 仲伟鉴[1] 

机构地区：[1]上海市疾病预防控制中心,上海200336

出　　处：《环境与职业医学》2006年第5期423-425,共3页Journal of Environmental and Occupational Medicine

摘　　要：[目的]用几种组织化学染色方法来探讨豚鼠皮肤黑色素反应,对不同染色法进行比较。[方法]利用多巴氧化酶法(DopA)、硫酸亚铁吸收法、亚铁氰化钾法和银浸染色法(Masson-fontana)对黑色素细胞进行染色。[结果]多巴氧化酶法,黑色素细胞呈黑色。硫酸亚铁吸收法,黑色素细胞和嗜银细胞呈暗绿色。亚铁氰化钾法,黑色素细胞和嗜银颗粒为蓝黑色。银浸染色法,黑色素细胞、亲银细胞和嗜铬细胞均呈黑色。[结论]多巴氧化酶法在几种方法中是最适合观察黑色素细胞数量和黑色素颗粒的方法。[ Objective ] To observe the reaction of melanin in guinea pig skin with several histochemical stare methods. [ Methods ] Melanocyte was stained with dopa oxidase （ DopA ）, ferrovitriol, ferropotassuim cyanide and Masson-fontana silver, respectively. [ Results ] Only melanocyte showed black with DopA： melanocyte and argentaffinocyte were sap green with ferrovitriol; either melanoeyte or argyrophilie grains showed black blue with ferropotassuim cyanide; melanocyte, argentaffinocyte and phaeochromocyte were black with Masson-fontana. [ Conclusion ] DopA is the most suitable method to observe the quantity of melanocyte and melanin granules.

关 键 词：黑色素 黑色素细胞 多巴氧化酶法 硫酸亚铁吸收法 亚铁氰化钾法 银浸染色法 

分 类 号：R114[医药卫生—卫生毒理学]