大鼠博落回总碱中毒心肌细胞凋亡的研究  

Apoptosis in Myocardial Cells after Macleaya Cordata total Alkaloids Poisoning in Rats

作　　者：张德雨[1] 朱建华[1] 赵伟志 吴茂旺[1] 胡永良[1] 程利宝[1] 李永宏[1] 

机构地区：[1]皖南医学院法医学系,安徽芜湖241001 [2]马钢医院病理科,安徽马鞍山242001

出　　处：《法医学杂志》2006年第5期330-332,F0004,共4页Journal of Forensic Medicine

基　　金：安徽省教育厅科研资助项目(K80143)

摘　　要：目的观察博落回总碱中毒后大鼠不同时间内心肌细胞凋亡情况,为博落回总碱中毒提供毒理学指标。方法以SD大鼠博落回总碱中毒为实验模型,通过HE染色及凋亡细胞检测技术对大鼠博落回总碱中毒后心肌病理变化进行研究,并对研究结果进行计算机病理学图像分析。结果大鼠在中毒后的不同时间段内,心肌细胞凋亡数高于正常对照组(P<0.01),且凋亡数在不同时间段差异有显著性。结论博落回总碱中毒在临床症状不明显及毒物分析难以检测的情况下,应用凋亡细胞检测技术可成功检测出心肌的病理学变化。Objective To observe pathological changes and apoptosis in rats myocardial cells after Macleaya cordata total alkaloids poisoning, and to provide some references for Macleaya cordata total alkaloids poisoning detection. Methods An experimental model of Macleaya cordata total alkaloids poisoning was established,and the technology of TUNEL staining was used.The results were analyzed by computer image analysis competitive system. Results Quantities of apoptosis in myocardial cells in poisoning groups were much more than those in the control groups at different tages （P〈0.01）. In addition the quantities of apoptosis were different after different poisoning duration. Conclusion Although clinical symptoms was not obvious and could not be detected by poison analysis.Pathological changes induced by Macleaya cordata total alkaloids could be found through the apoptosis detection.

关 键 词：博落回 生物碱 中毒 细胞凋亡 免疫组化 图像分析 

分 类 号：R542.2[医药卫生—心血管疾病]