Quantitative analysis of 3-dimensional root architecture based on image reconstruction and its application to research on phosphorus uptake in soybean  

作　　者：ZHU Tong lin FANG Suqin LI Zhiyuan LIU Yutao LIAO Hong YAN Xiaolong 

机构地区：[1]Root Biology Center, South China Agricultural University, Guangzhou 510642, China [2]Department of Computer Science, South China Agricultural University Guangzhou 510642, China

出　　处：《Chinese Science Bulletin》2006年第19期2351-2361,共11页

基　　金：This work was jointly supported by the National Key Basic Research Funds of China(Grant No.2005CB120902);the National Natural Science Foundation of China(Grant No.30230220);the McKnight Foundation Collaborative Crop Research Program(Grant No.05-780).

摘　　要：Quantification of 3-dimensional (3-D) plant root architecture is one of the most important approaches to investigating plant root growth and its function in nutrient acquisition and utilization. However, no effective methods have been reported hitherto to quantify 3-D root architecture parameters, making it difficult to further study the 3-D characteris- tics of the root system and its function. In the present study, we created a rapid algorithm to reconstruct 3-D root system images based on the basic structural features of such linear objects as roots, using 2-D root images taken by digital CCD cameras at multi- viewing angles. This method is very effective in the reconstruction of plant root system images, thus enabling us to obtain the digital model of 3-D root architecture and its 3-D skeleton, based on which some major root architecture parameters can be calculated. Using this method, we were able to ac- quire 3-D parameters of soybean root architecture whose root diameter was more than 0.3 mm, includ- ing tap root length, total root length, average basal root angle, ratio of root width to root depth, percent- age distribution of root length in different layers and root distribution in different 3-D regions of the growth medium. We also quantitatively analyzed the rela- tionship between different root architecture parame-ters and such plant nutrition parameters as soybean biomass and phosphorus (P) uptake. Our study may provide a new tool in studying the growth and nutri- tional functions of plant root systems.Quantification of 3-dimensional （3-D） plant root architecture is one of the most important approaches to investigating plant root growth and its function in nutrient acquisition and utilization. However, no effective methods have been reported hitherto to quantify 3-D root architecture parameters, making it difficult to further study the 3-D characteristics of the root system and its function. In the present study, we created a rapid algorithm to reconstruct 3-D root system images based on the basic structural features of such linear objects as roots, using 2-D root images taken by digital CCD cameras at multiviewing angles. This method is very effective in the reconstruction of plant root system images, thus enabling us to obtain the digital model of 3-D root architecture and its 3-D skeleton, based on which some major root architecture parameters can be calculated. Using this method, we were able to acquire 3-D parameters of soybean root architecture whose root diameter was more than 0.3 mm, including tap root length, total root length, average basal root angle, ratio of root width to root depth, percentage distribution of root length in different layers and root distribution in different 3-D regions of the growth medium. We also quantitatively analyzed the relationship between different root architecture parameters and such plant nutrition parameters as soybean biomass and phosphorus （P） uptake. Our study may provide a new tool in studying the growth and nutritional functions of plant root systems.

关 键 词：大豆 根系 图象再现 磷效率 定量分析 

分 类 号：S565.1[农业科学—作物学]