胃炎及十二指肠溃疡患儿胃窦胃泌素与生长抑素、增殖与凋亡的检测  

Expression of gastrin,somatostatin,PCNA and Fas-L in the mucosa of gastric antrum of children with chronic gastritis and duodenal ulcer

作　　者：谢小志[1] 王宗敏[1] 张海燕[1] 王兰[1] 高宝辉[1] 李雪梅[1] 胡伟国[1] 

机构地区：[1]温州医学院附属育英儿童医院胃肠学科,325027

出　　处：《中华儿科杂志》2006年第10期774-777,共4页Chinese Journal of Pediatrics

摘　　要：目的探讨胃窦组织中胃泌素(GAS)、生长抑素(SS)、增殖细胞核抗原(PCNA)、凋亡Fas基因配体(Fas-L)的免疫组化表达在小儿慢性胃炎、十二指肠溃疡发病机制中的意义。方法本组83例,根据病理诊断与胃镜下有无十二指肠溃疡将病例分为5组:A组,慢性浅表性胃炎,幽门螺杆菌阳性(Hp^+);B组,慢性浅表性胃炎,Hp^-;C组,十二指肠溃疡,Hp^+;D组,十二指肠溃疡,Hp^-;E组,正常对照组,Hp^-。应用免疫组化EnVision法,对每例进行GAS、SS、PCNA、Fas-L染色,比较各组阳性细胞数差异。结果GAS、SS的表达在A组、B组、C组、D组数值均高于E组,但差异无统计学意义;PCNA的表达在A组高于B组,差异有统计学意义(P<0.05);Fas-L的表达在各组间差异无统计学意义。结论在小儿慢性胃炎及十二指肠溃疡中,GAS、SS表达均有升高,GAS和SS升高可能在小儿慢性胃炎及十二指肠溃疡病因、防御机制中起一定作用;Hp感染促进胃窦黏膜腺上皮细胞的增殖。Objective Since applicaion of pediatric gastroscopy in the mid-nineteen nineties, there has been a trend that the prevalence rates of pediatric gastritis and duodenal ulcer （DU） are increasing. The diagnosed rate of pediatric gastritis has accounted for 85% -95% of the total number of children who received gastroscopy , and the rate of DU accounted for 8% -22%. Such a high rates of the diseases may influence the development of the children severely. However, the etiology and pathogenesis of pediatric chronic gastritis and DU have not been completely elucidated. The disordered gastrointestinal hormones play a crucial role in the pediatric chronic gastritis and DU. This study focused on the expression of gastrin （ GAS）, somatostatin （SS） in the mucosa of gastric antrum and PCNA and Fas-L in the sinus ventriculi and their possible roles in the pathogenesis of pediatric chronic gastritis and DU. Method The sinus ventriculi mucosal samples of 83 cases were collected via gastroscopic biopsy from the hospital during the recent two years and the cases were divided into five groups： group A, chronic superficial gastritis, Helicobacter pylori （Hp）^＋; group B, chronic superficial gastritis, Hp^- ; group C, DU, Hp^＋ ; Group D, DU, Hp^- ; Group E, normal sinus ventriculi mucosa, Hp^-. Immunohistochemical staining （En Vision） was carried out for GAS, SS, PCNA and Fas-L, and positive cells of each slide were counted （ ×400）. Statistically significant differences among groups for continuous data were assessed with the software SPSS10. 0. Results The expressions of GAS and SS in the groups A through E had no significant difference. The expression of PCNA in group A was significantly higher than that in group B （ P 〈 0. 05 ） , and no significant differences were found among the other groups. There were no significant differences in expressions of Fas-L among the five groups. Conclusion There seems to be an increasing tendency in the expressions of GAS and SS in children with chronic gastritis a

关 键 词：胃炎 十二指肠溃疡 胃泌素类 生长抑素 增殖细胞核抗原 膜糖蛋白类 

分 类 号：R725.7[医药卫生—儿科]