整合素β_3-粘着斑激酶信号途径的活化参与大鼠血管新生内膜的形成  

Integrin β_3-FAK pathway is involved in neointima formation

作　　者：温进坤[1] 韩梅[1] 程云会[1] 杨长春[1] 刘智敏[1] 

机构地区：[1]河北医科大学基础医学研究所河北省医学生物技术重点实验室,河北石家庄050017

出　　处：《中国病理生理杂志》2006年第10期1922-1925,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30570661);国家科技攻关计划项目(No.2002BA755C);河北省自然科学基金资助项目(No.C2006000814)

摘　　要：目的:观察整合素β3-粘着斑激酶(FAK)等信号分子表达和活化与血管新生内膜形成之间的关系。方法:用球囊剥脱法制备血管狭窄模型;用W estern印迹和免疫组化染色检测骨桥蛋白(OPN)、整合素β3和FAK等信号分子的蛋白水平及活化程度。结果:血管内皮剥脱后,OPN、整合素β3和FAK蛋白水平均在血管内皮剥脱后3 d明显增加;术后7 d时达高峰,其中升高的FAK以磷酸化形式为主;14-21 d时,有所回降,但仍高于正常。其表达峰值均出现在血管平滑肌细胞向内膜快速迁移和增殖期,而且伴有细胞外基质降解加快。结论:细胞迁移是一个细胞和细胞外基质相互作用的复杂过程,整合素β3-FAK信号途径在维系该过程的协调统一方面具有重要作用。AIM： To investigate whether the expression of integrin β3 and the activation of focal adhesion kinase （FAK） are involved in neointima formation after de - endothelium. METHODS： The model of intima hyperplasia was prepared by balloon injury. The levels of osteopontin （OPN）, integrin β3 and FAK in vascular tissue were detected by Western blotting and immunohistochemistry. RESULTS： There were similar expression patterns in OPN, integrin β3 and FAK following balloon injury. The levels of three proteins were markedly increased 3 days after operation and reached the peak at 7th day. The increased FAK was mainly the phosphorylated form. The migration and proliferation of vascular smooth muscle cells was associated with the increase in the expression of integrin β3 and FAK, and was parallel with rapid turnover of extracellular matrix （ECM）. CONCLUSION： The interaction of cells with ECM mediated by OPN and integrin β3 is essential for migration. The integrin β3 - FAK pathway is involved in neointima formation.

关 键 词：血管内膜 细胞外基质 结合素类 粘着斑激酶 

分 类 号：R543.3[医药卫生—心血管疾病]