椪柑上胚轴离体培养与植株再生研究初报  

Study on In Vitro Culture and Plant Regeneration in Citrus Reticulata Blanco Epicotyl

作　　者：鲁旭东[1,2] 刘华英[1] 萧浪涛[1] 

机构地区：[1]湖南农业大学植物激素重点实验室 [2]孝感学院生命科学技术学院,湖北孝感432000

出　　处：《中国农学通报》2006年第10期67-69,共3页Chinese Agricultural Science Bulletin

基　　金：教育部青年骨干教师基金"两系杂交稻内源激素代谢研究"(2000;科教司65号)

摘　　要：椪柑无菌苗上胚轴为实验材料进行离体培养,研究了不同种类的激素及其组合对椪柑上胚轴出芽及再生芽生根的影响。结果表明:在MT+6-BA2mg/L培养基上,上胚轴垂直接种时,出芽率可达100%。在MT培养基上单独使用ABA0.2mg/L、GA30.4mg/L或GA30.4mg/L+6-BA2mg/L对芽再生均有一定的抑制作用,但ABA0.2mg/L+6-BA2mg/L则对芽再生有一定的促进作用。1/2MT+NAA0.5mg/L对再生芽生根效果最好,生根率为87.5%。We studied effects of different hormones and combination on buding and rootting of Citrus reticulata Blanco epicotyl in tissue culture. The results showed 100% buding ratio in MT＋6-BA2mg/L when epicotyl was vertical inserted. The buding was restrain in MT medium with only ABA0.2 mg/L, GA30.4 mg/L and GA30.4 mg/L＋6-BA2 mg/L. But buding was promoted in MT medium with ABA0.2 mg/ L＋ 6-BA2 mg/L.The rootting was the best（87.5% rootting ratio ） in 1/2MT＋NAA0.5 mg/L.

关 键 词：柑 上胚轴 离体培养 

分 类 号：S687.3[农业科学—观赏园艺]