T-DNA诱发的小麦强分蘖突变体研究初报  

Analysis of A Wheat Mutant with High Tillering Created by T-DNA

作　　者：何道一[1] 王旭明[1] 陈楚[1] 

机构地区：[1]淮北煤炭师范学院生物系,安徽淮北235000

出　　处：《中国农学通报》2006年第10期89-92,共4页Chinese Agricultural Science Bulletin

基　　金：安徽省教育厅自然科学研究项目"T-DNA插入诱发的小麦强分蘖突变体分析及相关基因的克隆"(2005KJ193)

摘　　要：利用农杆菌介导的小麦活体转化技术转化多个小麦品种,得到一定数量的小麦转化体植株。在小麦品种鲁麦21的遗传转化体T0代植株中发现一强分蘖突变体,通过PCR技术和Southern杂交技术证明该突变体含有T-DNA序列;该突变体表现为单基因隐性突变,在突变体T3代中均能通过PCR扩增到T-DNA内的序列,表明该突变体为T-DNA插入诱发的突变体。通过对该突变体初步分析表明突变基因与温度应答有关,随温度的降低,突变体分蘖速度加快。Several wheat cultivars were transformed in planta by Agrobacterium tumefaciens and numbers of transformants were gained. A mutant with high tillering was found in the transmants of the wheat cultivar Lumai 21. The mutant was identified with part of T-DNA sequence by PCR and Southern blot and displayed a recessive gene mutation. Mutant plants of T3 were found with part of T-DNA sequence by PCR. This indicated the mutant was created by T-DNA. Primary analysis suggested that the mutation gene is relative to plant response to temperature. Tiller number of the mutant increased much more than that of the wild parent with temperature decrease.

关 键 词：小麦 突变体 分蘖 T-DNA 转化 

分 类 号：S512.1[农业科学—作物学] Q319.31[生物学—遗传学]