血管紧张素Ⅱ及受体2诱导新生鼠肾小管上皮细胞凋亡的研究  

Induced apoptosis of renal tubular cells from neonate rats by angiotensinⅡand AT_2 receptor

作　　者：李冬[1] 李国华[1] 徐立新[1] 王晓炜[2] 刘佳[2] 

机构地区：[1]大连医科大学第二临床学院儿科,辽宁大连116027 [2]大连医科大学生物学教研室,辽宁大连116027

出　　处：《大连医科大学学报》2006年第5期353-356,共4页Journal of Dalian Medical University

摘　　要：[目的]研究血管紧张素Ⅱ和受体2对肾小管上皮细胞凋亡诱导的作用.[方法]取新生大鼠肾小管上皮细胞体外培养,传代后分别加入不同浓度的血管紧张素Ⅱ和AT2受体激动剂CGP-42112A,孵育24 h, 流式细胞仪测定凋亡细胞比值, RT-PCR半定量检测AT2受体mRNA表达.[结果]血管紧张素Ⅱ以10^-5mol/L、10^-6mol/L、10^-7mol/L、10^-8mol/L浓度加入培养细胞24 h后,凋亡细胞比值分别为（21.73±1.25）%、（18.65±0.49）%、（17.29±0.67）%、（15.98±0.71）%,均显著增高于对照组（6.89±1.17）%,（P＜0.001）.CGP-42112A以10^-5～10^-8mol/L浓度作用于细胞后凋亡细胞值分别为（22.32±2.69）%、（19.17±1.63）%、（17.98±1.96）%、（16.06±1.49）%,均显著高于对照组（7.04±0.61）%,（P＜0.001）,凋亡细胞数与2种药物均呈剂量依赖性.RT-PCR半定量分析,两种药物作用于细胞后,随着浓度的逐渐增加,AT2受体mRNA的表达均逐渐增强.[结论]血管紧张素Ⅱ和 AT2受体兴奋剂均可诱导肾小管上皮细胞凋亡,血管紧张素Ⅱ诱导细胞凋亡可能通过AT2受体途径实现.[Objective] To invest the role of angiotensin Ⅱ and AT2 receptor in apoptosis of renal tubular cells. [Methods] In vitro neonate Wistar rat＇s renal tubular cells culture, Angiotensin Ⅱ （Ang Ⅱ） or AT2 receptor agonist CGP - 42112A in different doses were diffused separately, and at 24h, cells were analyzed by flow cytometry analysis of apoptosis, AT2 receptor mRNA expression was measured by RT - PCR. [Results] In the groups with the diffusion of Ang Ⅱ at 10^-5mol/L, 10^-6mol/L, 10^-7mol/L and 10^-8mol/L, apoptotic cell rations were （21.73±1.25） %, （18.65±0.49） %, （17.29±0.67） % and （15.98±0.71） % respectively, being higher than that in group （6.89±1.17）%, （P 〈 0.001）. In the groups with diffusion at 10^-5mol/L, 10^-6mol/L, 10^-7mol/L and 10^-8mol/L of CGP - 42112A, apoptotic cell rastions were that （22.32±2.69）%, （19.17±1.63）%, （17.98±1.96）% and （16.06±1.49） % respectively, being higher than that of （7.04±0.61） %, （P 〈 0.001）, a dose dependent manner was found, but difference was insignificant between Ang Ⅱ and CGP-42112A groups. RT - PCR results shown that the AT2 receptor mRNA levels were up-regulated by both of CGP - 42112A and Ang Ⅱ. [Conclusions] Angiotensin Ⅱ and CGP - 42112A may up - regulated the renal tubular cells＇ apoptotic rations. The apoptosis was induced by Angiotensin Ⅱ maybe via the way of AT2 receptor.

关 键 词：血管紧张素Ⅱ CGP-42112A 凋亡 

分 类 号：R726.9[医药卫生—儿科]