一氧化氮在大剂量顺铂所致大鼠肾损伤早期中的变化  

Function of nitric oxide in high dose cisplatin induced acute renal failure in rats

作　　者：张建青[1] 赵文安[1] 刘霏[1] 于子涵[1] 杜宣[1] 刘艳华[1] 王晓倩[1] 张锦程[1] 滕忠义[1] 刘星[1] 姜妙娜[2] 李墨林[2] 

机构地区：[1]大连医科大学临床医学 [2]大连医科大学病理生理学教研室,辽宁大连116027

出　　处：《大连医科大学学报》2006年第5期381-383,共3页Journal of Dalian Medical University

摘　　要：[目的]观察一氧化氮(NO)在大剂量顺铂所致大鼠急性肾功能衰竭早期中的变化。[方法]SD大鼠18只,雌雄各半,随机分为顺铂(DDP)用药组和生理盐水对照组,每组9只。各组腹腔内一次性注射顺铂10 mg/kg或等量生理盐水后,动态观察大鼠体重的变化,用药后6 h取各组肾脏计算肾系数,并用生化法测定血清尿素氮和肾皮质匀浆中NO的水平,统计学分析。[结果]大剂量顺铂用药6 h后,大鼠体重开始明显降低,用药后3 d大鼠死亡。用药后6 h血清BUN水平两组比较无显著差异(P>0.05),顺铂用药组为(18.41±4.7)mg/dL,对照组为(18.44±4.03)mg/dL;但用药后6 h肾系数和肾皮质匀浆NO水平两组比较差异显著(P<0.05),顺铂用药组分别为(9.39±0.78)和(24.79±11.27)μmoL/gprot,对照组分别为(8.34±0.60)和(5.27±2.4)μmoL/gprot。[结论]10 mg/kg顺铂可引起大鼠急性肾功能衰竭并致死,NO在肾损伤早期发挥重要作用。[Objective] To investigate the level of nitric oxide （NO） in high dose cisplatin induced acute renal failure in rats. [Methods] SD rats were received cisplatin （10 mg/kg） intraperitoneally and the same volume of natural saline was given as control. Body weight of rats was measured before and 6 h, 48 h, 72 h after the administrations. Renal coefficient was measured 6 h after the injection, The levels of blood urea nitrogen （BUN）, protein contents and NO contents in renal cortex tissue were measured 6 hours after administrations. [Results] Body weight of rats decreased significantly after cisplatin administration and the rats died within 3 d. There are no significant differences in BUN between cisplatin group （18.41±4.7）mg/dL and control group（18.44±4.03）mg/dL 6 hours after administrations （P 〉 0.05）. But the renal coefficient and the levels of NO in renal cortex tissue are （9.39±0.78） and （24.79±11.27） μmoL/gprot respectively in cisplatin group, significantly higher than those （8.34±0.60） and （5.27±2.4）μmoL/ gprot respectively in control group 6 hours after administrations （P 〈 0.05）. [Conclusions] Acute renal failure induced by high dose of cisplatin （10 mg/kg） can cause rats dead. NO plays an important role in the early stage of acute renal failure.

关 键 词：大剂量顺铂 肾功能衰竭 SD大鼠 动物模型 一氧化氮(NO) 

分 类 号：R692[医药卫生—泌尿科学]