木那格葡萄快速繁殖及愈伤组织的诱导和继代  

Research on Rapid Propagation and Callus Inducing and Maintenance of Grape Cultivar‘Munage’of Vitis vinifera

作　　者：吕惠平[1] 王起才[1] 马纪[1] 

机构地区：[1]新疆生物资源基因工程重点实验室新疆大学生命科学与技术学院,新疆乌鲁木齐830046

出　　处：《生物技术》2006年第5期75-77,共3页Biotechnology

基　　金：科技部重大基础研究前期研究专项(No.2003CCA01000);新疆生物资源基因工程重点实验室开放课题(No.XJDX0201-200403)资助

摘　　要：研究了木那格葡萄快速繁殖并确定了木那格葡萄愈伤组织诱导和继代的适宜条件。将木那格葡萄种子无菌处理,获得无菌幼苗,以单芽茎段为外植体,对木那格葡萄快速繁殖条件进行优化；以叶片为外植体,确定愈伤组织诱导和继代的适宜条件。结果表明：使用70%乙醇灭菌30s,0．1%升汞灭菌3min,使用浸湿的滤纸培养,适宜木那格葡萄种子萌发,萌发率为70%；适宜单芽茎段不定芽再生的培养基为B5+6-BA 0．05mg/L+IBA 0．3mg/L+蔗糖30g/L,不定芽增殖倍数为1．25；适宜不定芽生根的培养基为B5+IBA 0．2mg/L+蔗糖30g/L,平均单芽生根数为5．09,平均根长2．97cm；适宜愈伤诱导的培养基为B5+6-BA1．5mg/ L+NAA 0．2mg/L+蔗糖30g/L,愈伤诱导率为100%；适宜愈伤继代的培养基为B5+6-BA 1mg/L+2,4-D 0．1mg/L+蔗糖30g/L。Rapid propagation and callus inducing and maintenance of grape cultivar ＇ Munnge＇ were researched. The results showed that the condition suitable for the grape seeds germination was that seeds surface sterilized with 70% ethanol for 30s and with 0.1% ~ for 3rain and then cultured on sterilized water soaked filter paper. Stem segments with single bud were used as explants to study rapid propagation of the grape. Leaves were used as explants to induce and maintain callus. The suitable culture medium for adventitious bud inducing was B5 ＋ 6 - BA 0.05mg/L＋ IBA 0.3mg/L ＋ sucrose 30g/L, for root inducing was B5 ＋ IBA 0.2mg/L ＋ sucrose 30g/L, for callus inducing was B5 ＋ 6 - BA 1.5mg/L ＋ NAb. 0.2mg/L ＋ sucrose 30g/L, for callus maintenance was/35 ＋ 6- BA lmg/L ＋ 2, 4 - D 0. l mg/L ＋ sucrose 30g/L. The highest adventitious bud multiplication rate obtained in this study was 1.25. The average rooting rate was 5.09 roots per planflet. The average root length was 2.97cm, and the callus inducing rate was 100%.

关 键 词：葡萄 木那格 快速繁殖 愈伤组织 

分 类 号：Q943.1[生物学—植物学]