IL-8诱导血管内皮细胞迁移的实验研究  

Experimental Study on the Migration of Vascular Endothelial Cells Stimulated by IL-8

作　　者：邹敏君[1] 刘肖珩[1] 李毅[1] 赖怡[1] 

机构地区：[1]四川大学华西医学中心生物医学工程研究室,成都610041

出　　处：《生物医学工程学杂志》2006年第5期1013-1016,共4页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(30570450);四川省杰出青年基金资助项目(06ZQ026-009)

摘　　要：为了探讨不同浓度IL-8诱导的血管内皮细胞迁移从而为进一步研究IL-8引起血管内皮细胞迁移的分子机理选择最佳的IL-8浓度。采用内皮细胞transw e ll小室迁移率分析法和刮除-迁移分析法,考察IL-8对血管内皮细胞迁移的影响。结果表明,各种浓度的IL-8均可促进血管内皮细胞迁移,其中以IL-8浓度为100 ng/m l时内皮细胞迁移率最高。To investigate the influence of different concentrations of IL-8 on the migration of vascular endothelial cells and find out the best IL-8 concentration, the transwell chamber motility assay and the scrape motility assay were applied to observe the migration of vascular endothelial cells induced by IL-8, The results demonstrated that the migration of vascular endothelial cells was increased significantly under different IL-8 concentrations, while the best effect occurred when IL-8 concentration was 100 ng/ml.

关 键 词：IL-8 内皮细胞 细胞迁移 

分 类 号：R392[医药卫生—免疫学]