碳素材料表面吸附蛋白的红外光谱分析  

Analysis of FT-IR-ATR Spectra of Serum Proteins Adsorbed on Carbonaceous Materials

作　　者：李伯刚[1] 那娟娟[1] 殷杰[1] 尹光福[1] 

机构地区：[1]四川大学材料科学与工程学院,成都610065

出　　处：《生物医学工程学杂志》2006年第5期1052-1055,共4页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(39870232)

摘　　要：为探讨类金刚石薄膜(DLC)、金刚石薄膜(DF)和石墨不同血浆蛋白吸附特性的内在原因,对人血白蛋白(HSA)和纤维蛋白原(HFG)在三种碳素材料表面吸附前后进行了红外光谱分析。结果显示:通过氨基,在HSA与DLC、HFG与DF、HFG与石墨之间形成了氢键,从而有力地支持并合理地解释了DLC具有较高的HSA吸附活性、DF和石墨则优先吸附HFG的前期研究结论。To clarify the reason causing difference of serum proteins adsorbability on different carbonaceous materials, FT-IR-ATR spectra of human serum albumln（HSA） and human serum fibrinogen （HFG） before and after adsorbing on diamond like carbon film （DLC）,diamond film （DF） and graphite were analyzed. It has been shown that there are hydrogen bond because of -NH at the interfaces of HSA-DLC,HFG-DF and HFG-graphite. Based on the results, earlier research conclusion that the adsorbability of HSA on DLC higher than that on DF and graphite, but on DF and graphite the adsorption of HFG takes precedence can be explained rationally.

关 键 词：类金刚石薄膜(DLC) 金刚石薄膜(DF) 石墨 蛋白吸附 氢键 

分 类 号：R318.08[医药卫生—生物医学工程]